高效液相色谱法测定单叶化香树果序中没食子酸含量  

Content Determination of Gallic Acid in Infructescence of Platycarya Strobilacea

作　　者：刘金坤[1] 应敏 王琴[1] 敖利[3] 吴斌[1] Liu Jinkun;Ying Min;Wang Qin;Ao Li;Wu Bin(Basic Research Laboratory,Chongqing HospitalofTraditionalChinese Medicine,Chongqing,China 40002;Chongqing CancerHospital,Chongqing,China 400030;Department of Pharmacy,Chongqing Hospital of Traditional Chinese Medicine,Chongqing,China 400021)

机构地区：[1]重庆市中医院中医药基础研究室,重庆400021 [2]重庆大学附属肿瘤医院,重庆400030 [3]重庆市中医院药剂科,重庆400021

出　　处：《中国药业》2018年第6期4-7,共4页China Pharmaceuticals

基　　金：重庆市基础科学与前沿技术研究项目[cstc2017jcyj AX0369];重庆市卫生计生委基础中医药项目[ZY201702038];重庆市基本科研业务费计划项目[cstc2015jbky330025011]

摘　　要：目的建立测定单叶化香树果序中没食子酸含量的高效液相色谱(HPLC)法。方法色谱柱采用Diamonsil~C_(18)(2)柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.2%磷酸水溶液(3∶97),检测波长为270 nm,进样量为10μL,流速为1.0 m L/min。结果没食子酸进样量在0.137 5~11.000 0μg范围内与峰面积线性关系良好,r=0.999 8,平均回收率为100.99%,RSD为1.13%(n=6)。结论该方法准确、简便、易行,可用于检测单叶化香树果序中没食子酸的含量。Objective To establish an HPLC method for gallic acid ininfructescence of Platycarya strobilacea.Methods Diamonsil R C18(2)column(250mm×4.6mm,5μm)was adopted with the mobile phase of methanol-0.2%phosphoric acid(3∶97),the detection wavelength was 270nm,the sample size was 10μL,and the flow ratewas1.0mL/min.Results The gallic acid was linear in the range of0.1375-11.0000μg(r=0.9998),the average recovery was 100.99%,and RSD was1.13%(n=6).Conclusion The method is accurate,simple and easy,which is suitable for content determination of gallicacid in infructescence of Platycarya strobilacea.

关 键 词：单叶化香树 果序 没食子酸 高效液相色谱法 含量测定 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]