富硒治疗对大鼠急性一氧化碳中毒性脑病的作用  

Protective Effect on Brain Tissue Damage of Rats by Selenium-rich Treatment after Acute Carbon Monoxide Poisoning

作　　者：李金兰 刘群会 曹学兵[2] 贾敏 谭明会 聂淑科 陈贵勤 LI Un-Lan;LIU Qirn-hui;CAO Xue-bing;MA Min;TAN Ming-hui;NIE Shu-ke;CHEN Gui-qin(Department of Neurology,Enshi Tujia and Miao Autonomous Prefecture Center Hospital,Hubei 445000,China;Department of Neurology,Union Hospital,Tongi Medical College,Huazhong University of Science ad Technology,Wuhan 430032,China;Department of Neurology I Division,Renmin Hospital of Wuhan University,Wuhaa 430060,Chia)

机构地区：[1]恩施土家族苗族自治州中心医院神经内科,湖北恩施445000 [2]华中科技大学同济医学院附属协和医院神经内科,武汉430032 [3]武汉大学人民医院神经内科Ⅰ科,武汉430060

出　　处：《神经损伤与功能重建》2018年第1期1-5,共5页Neural Injury and Functional Reconstruction

摘　　要：目的:研究富硒治疗对急性一氧化碳(CO)中毒性脑病模型大鼠的作用及其作用机制。方法:60只SD大鼠随机分为正常对照组(BC组)、急性CO中毒组(CO组)、低硒治疗组(L-Se组)、中硒治疗组(M-Se组)、高硒治疗组(H-Se组)各12只。腹腔注射CO制备急性CO中毒模型。造模前30 d及造模后每天分别给予各组纯水或不同剂量富硒药物灌胃治疗。造模后第7天、第14天分别取脑及外周脏器,HE染色观察组织变化;对脑皮质行小白蛋白(PV)免疫组化染色,Western Blotting检测皮质PV和Caspase-3的蛋白表达水平。结果:硒对急性CO中毒大鼠模型的肝脏、肾脏无明显的毒性损伤。H-Se组在造模后第7天、第14天的皮质PV阳性细胞数均高于CO组(P<0.05);L-Se组、M-Se组在造模后第7天、第14天的皮质PV阳性细胞数与CO组无明显差异(P>0.05);H-Se组的PV阳性细胞数与BC组无明显差异(P>0.05)。H-Se组在造模后第7天、第14天的PV表达量高于CO组(P<0.05),Caspase-3蛋白表达低于CO组(P<0.05),与BC组无明显差异(P>0.05);L-Se组、M-Se组在造模后第7天、第14天的PV和Caspase-3蛋白表达量与CO组无明显差异(P>0.05)。结论:高剂量的硒治疗可明显上调PV,减少Caspase-3的活化。Objective:To investigate the potential mechanism and protective effect against brain tissue damage i rats by selenium-rich treatment after acute carbon monoxide(CO)poisoning.Methods:Atotal of 60 SDrats were randomly assigned to groups blank control(BC),CO poisoning(CO),low dose selenium treatment(L-Se),medium dose selenium treatment group(M-Se),and high dose selenium treatment group(H-Se),with 12 rats per group.Rats were intraperitoneally injected with CO to establish animal models of acute CO poisoning while those rn the control group were injected with an equal volime of ai.30 days before and every day after generating model,rats were treated with H2O or various doses of selenium-rich drug.7 and 14 days afer generating model,cerebral cortical tissue and peripheral tissues were collected,HE stining was performed on peripheral tissue organs to observe stmctural changes.Immmiohistochemistry and Western blot were performed on cortical tissue to assess levels of parvalbumm(PV)and Caspase-3 protein expression.Results:CO poisoned rat pempheral tissues sustamed no significant injiuy from selenium treatment.The average PV positive cells rn the 7-day and 14-day poisoning groups were significantly reduced compared to the H-Se goup(P<0.05);reduction ni the 7-day and 14-day poisoning groups was slightly significant compared to those i the L-Se group and M-Se group(P 0.05).There was no significant difference i PV positive cells between the BC group and H-Se group.PV level i the 7-day and 14-day H-Se treatment groups was significantly higher than that i the CO group(i><0.05)Caspase-3 level was lower than the CO group(P<0.05).There was no significant difference in PV and Caspase-3 level between the 7-day and 14-day H-Se treatment groups and the BC group(i^>0.05).There was no significant difference i PV and Caspase-3 level between groups L-Se,M-se and CO group at 7-day and 14-day(P>0.05).Conclusion:High doses of selenium treatment can markedly increase the level of PV and reduce the activation of Caspase-3.

关 键 词：急性一氧化碳中毒 小白蛋白 CASPASE-3 硒 钙超载 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.05[医药卫生—临床医学]