miR-34a调控MSR1蛋白对前列腺癌细胞迁移和侵袭的影响  

Effects of miR-34 on migration and invasion of prostate cancer cells by MSR1 protein

作　　者：韩兴涛[1] 杨凌博 魏澎涛[1] 李小辉 孙建涛[1] 杨锦建[2] HAN Xing-Tao;YANG Ling-Bo;WEI Peng-Tao;LI Xiao-Hui;SUN Jian-Tao;YANG Jin-Jian(Department of Urology Luoyang Central Hospital,Luoyang 471000,China)

机构地区：[1]洛阳市中心医院泌尿外科,洛阳471000 [2]郑州大学第一附属医院泌尿外科,郑州450052

出　　处：《中国免疫学杂志》2018年第4期544-548,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(No.81570685)

摘　　要：目的:探讨miR-34a通过MSR1对前列腺细胞迁移和侵袭的影响。方法:Western blot检测前列腺癌PC3细胞中MSR1表达情况;q PCR检测前列腺癌PC3细胞中miR-34a的表达情况,使用双荧光素酶实验检测miR-34a和MSR1之间的相关性;Transwell侵袭实验检测miR-34a和MSR1对前列腺癌细胞侵袭能力的影响;划痕愈合实验检测miR-34a和MSR1对前列腺癌细胞迁移能力的影响。结果:Western blot检测前列腺癌PC3细胞中MSR1的表达水平相对较高;q PCR检测前列腺癌PC3细胞中miR-34a的表达水平相对较低;双荧光素酶检测miR-34a和MSR1之间有直接的结合位点,miR-34a可以调控MSR1的表达水平,划痕愈合试验和Transwell侵袭试验检测过表达miR-34a后前列腺癌PC3细胞的迁移和侵袭能力受到相应的抑制,过表达MSR1后可以逆转miR-34a对前列腺癌PC3细胞的迁移和侵袭能力的抑制作用。结论:miR-34a可以通过MSR1蛋白来调控前列腺癌细胞的迁移和侵袭行为。Objective:To investigate the effect of miR-34a on the migration and invasion of prostatic cell line through MSR1.Methods:Western blot was used to detect the expression of MSR1 in prostate cancer cell lines.qPCR was used to detect the expression of miR-34a in prostate cancer cell lines.The correlation between miR-34a and MSR1 was examined by double luciferase assay.Transwell invasion assay was used to detect the effect of miR-34a and MSR1 on the invasion ability of prostate cancer cells.The effects of miR-34a and MSR1 on the fine migration ability of prostate cancer were examined by scratch healing.Results:The expression level of MSR1 in PC3 cells was relatively high by Western blot.The expression level of miR-34a in PC3 cells was relatively low by qPCR.Double luciferase assay showed a direct binding site between miR-34a and MSR1,MiR-34a could regulate the expression level of MSR1,Scaffold Healing Test and Transwell Invasion Test were used to detect the migration and invasion of PC3 cells after overexpressing miR-34a.Overexpression of MSR1 could reverse the inhibitory effect of miR-34a on the migration and invasion of PC3 cells.Conclusion:miR-34a can regulate the migration and invasion of prostate cancer cells through MSR1 protein.

关 键 词：MIR-34A 前列腺癌 MSR1 侵袭能力 

分 类 号：R737.25[医药卫生—肿瘤]