HBV相关原发性肝癌患者血清B7-H3与IL-21的表达及其临床意义  

Expression and clinical significance of B7-H3 and IL-21 in serum of patients with HBV associated hepatocellular carcinoma

作　　者：李成德[1] 全毅[2] 蔡健梅[1] 吴小文[1] 申学基[1] 阮德斌 张琳[3] 李海珠[1] LI Cheng-De;QUAN Yi;CAI Jian-Mei;WU Xiao-Wen;SHEN Xue-Ji;RUAN De-Bin;ZHANG Lin;LI Hai-Zhu(Department of Clinical Laboratory,First People′s Hospital of Zhaoqing City,Zhaoqing 526020,China)

机构地区：[1]广东省肇庆市第一人民医院检验科,肇庆526020 [2]广东省肇庆市第一人民医院肿瘤介入科,肇庆526020 [3]广东省肇庆市第一人民医院消化内科,肇庆526020

出　　处：《中国免疫学杂志》2018年第4期569-575,共7页Chinese Journal of Immunology

基　　金：广东省肇庆市科技创新计划项目(2014E184)

摘　　要：目的:探讨血清B7-H3与IL-21在HBV相关原发性肝癌患者中的表达及临床意义。方法:收集HBV相关肝病121例,其中原发性肝癌50例作为肝癌组,良性组71例包括急性肝炎12例、慢性中至重度肝炎21例,肝硬化代偿期20例,肝硬化失代偿期18例;同期20例体检健康人员作为正常组。B7-H3和IL-21采用ELISA法检测,HBV-DNA定量运用实时荧光PCR检测。结果:肝癌患者血清B7-H3和IL-21含量分别为(207.60±57.16)ng/ml和(2 357.28±805.01)pg/ml,均高于正常组(P<0.001)。相对正常对照而言,良性组中不同临床类型患者二者含量均升高明显(P<0.001)。在HBV相关肝病患者血清中,B7-H3与IL-21呈正相关;B7-H3与HBV DNA无相关性,而IL-21与HBV DNA是否复制有关,但与其复制水平高低无关。结论:B7-H3、IL-21在HBV相关肝癌患者血清中表达水平高。机体B7-H3、IL-21持续地高表达可能与患者疾病的发展、预后有关。Gathering 121 cases of HBV-related patients,50 cases of primary hepatic carcinoma of them were considered as hepatic carcinoma group,71 cases of benign group including 12 cases with acute hepatitis,21 cases of chronic moderate to severe hepatitis,20 cases of compensatory phase cirrhosis,18 cases of decompensated cirrhosis and 20 cases of healthy persons in the same period as normal control.The content of serum B7-H3 and IL-21 were detected by ELISA.HBV DNA quantitative results were analyzed by Quantitative Real-time PCR.Results:The levels of B7-H3 and IL-21 in patients with primary hepatic carcinoma were(207.60±57.16)ng/ml and(2 357.28±805.01)pg/ml,respectively,which were significantly higher than those of the normal control subjects(P<0.001).Comparison with the normal control subjects,the content of B7-H3 and IL-21 in serum of patients with different clinical types in the benign group increased significantly(P<0.001).B7-H3 and IL-21 were positively associated with each other in serum of patients with HBV-related primary liver cancer.The expression of sB7-H3 was not significantly correlated with the degree of HBV DNA replication.The expression of IL-21 was correlated with HBV DNA replication in patients with HBV associated hepatocellular carcinoma,but was not significantly correlated with the degree of HBV DNA replication.Conclusion:HBV-related primary hepatic carcinoma express sB7-H3 and IL-21 with high level.The continuous high expression of sB7-H3 and IL-21 in the body may be related to the development and prognosis of the patients.

关 键 词：原发性肝癌 肝炎 乙型 可溶型B7-H3 IL-21 

分 类 号：R735.7[医药卫生—肿瘤] R512.62[医药卫生—临床医学]