幼兔CO_2人工气腹模型的建立  

Establishment of Laparoscopic Carbon Dioxide Pneumoperitoneum Model in Young Rabbits

作　　者：刘扬 李贵斌[1] 王春燕 邱云[1] Liu Yang;Li Guibin;Wang Chunyan(Department of Pediatric Surgery,Fifth Central Hospital of Tianjin,Tianjin 300450,China)

机构地区：[1]天津市第五中心医院小儿外科,天津300450

出　　处：《中国微创外科杂志》2018年第2期148-150,共3页Chinese Journal of Minimally Invasive Surgery

基　　金：天津市卫计委科技基金项目(2015KZ017);天津市滨海新区卫计委科技资金重点项目(2015BWKZ002)

摘　　要：目的构建一种实用稳定的CO_2人工气腹动物模型,模拟新生儿腹腔镜手术环境。方法 32只健康新西兰幼兔(3周龄,体重0.8~1 kg),随机分为4组,每组8只:对照组,单纯腹腔麻醉;开腹组,麻醉后行开腹手术暴露腹腔;低压气腹组,腹腔气腹压力维持于6 mm Hg;高压气腹组:腹腔气腹压力维持于12 mm Hg。采用10%水合氯醛3 ml/kg腹腔麻醉,追加采用4%水合氯醛1 ml/kg。麻醉时间持续4 h。气腹组采用精密电子压力计控制CO_2输入的方法维持目标气腹压力。结果应用精密电子压力计可以维持理想的气腹压力。各组幼兔围手术期心率、呼吸频率比较均无统计学差异(P>0.05),术后24 h内进食量恢复正常,术后7 d切口拆线,均一期愈合,未出现感染、出血及死亡。结论采用幼兔作为动物模型、精密电子压力计维持CO_2气腹压力的方法,可成功建立用于研究新生儿腹腔镜手术的CO_2人工气腹模型。Objective To establish a practical and stable model of CO 2 pneumoperitoneum and to provide experimental basis for clinical laparoscopic surgery in infants by simulating the newborn laparoscopic environment. Methods Thirty-two healthy young rabbits were randomly divided into four groups,which were control group,open group(abdomen surgery after anesthesia),pneumoperitoneum low pressure group(abdominal pressure maintained at 6 mm Hg)and pneumoperitoneum high pressure group(abdominal pressure maintained at 12 mm Hg).The 10%chloral hydrate 3 ml/kg was injected in the abdomen for anesthesia,and 4%chloral hydrate 1 ml/mg was added to confirm the effect of anesthesia.The anesthesia time lasted for 4 hours.A sophisticated electronic pressure gauge was used to control the input of CO 2 and maintain the target pneumoperitoneum pressure in the group of CO 2 pneumoperitoneum model. Results Application of precision electronic pressure gauge accurately maintained pneumoperitoneum pressure.In addition,there was no stastistically significant difference about the heart rate and respiratory rate amorg the 4 groups(P>0.05).The food intake returned normal in 24 hours after surgery.The incision was healed by first intention to remove the stitches 7 days after operation.There were no infection,hemorrhage or death occurred in all the 4 groups. Conclusion A model of artificial pneumoperitoneum for infant laparoscopic surgery can be successfully established by using rabbit as animal model and precision electronic manometer to maintain CO 2 pneumoperitoneum pressure.

关 键 词：腹腔镜手术 CO2气腹 幼兔 新生儿 

分 类 号：R-332[医药卫生] R726.5