针刺对脑缺血再灌注大鼠梗死灶边缘区顶叶皮质TLR9、TNF-α、IRF7和IFN-β的mRNA表达的影响  

Effect of acupuncture on the expression of TLR9, TNF-α, IRF7 and IFN-β mRNA in parietal cortex of cerebral infarction in rats with cerebral ischemia reperfusion

作　　者：许军峰[1,2] 张浪 田骞 郭士明 李文博[5] XU Junfeng;ZHANG Lang;TIAN Qian;GUO Shiming;LI Wenbo(Department of Special Need Acupuncture and Moxibustion,The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;Post-doctoral Mobile Research Center,China Academy of Traditional Chinese Medicine,Beijing 100700,China;Zichang People’s Hospital of Shaanxi Province,Zichang 717300,China;Tianjin Dongli District Chinese Medicine Hospital,Tianjin 300399,China;Tianjing University of Traditional Chinese Medicine,Tianjin 300193,China)

机构地区：[1]天津中医药大学第一附属医院针灸特需科,天津300193 [2]中国中医科学院博士后科研流动站,北京100700 [3]陕西省子长县人民医院,子长717300 [4]天津市东丽区中医院,天津300399 [5]天津中医药大学,天津300193

出　　处：《天津中医药》2018年第4期286-288,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家中医药管理局国家中医临床研究基地业务建设科研专项课题(JDZX2015015);天津中医药大学第一附属医院拓新工程课题(201202)

摘　　要：[目的]检测脑缺血再灌注模型早期梗死灶边缘区Toll样受体9(TLR9)、肿瘤坏死因子-α(TNF-α)、干扰素调节因子7(IRF7)、干扰素-β(IFN-β)的m RNA表达,阐明针刺对脑梗死的TLR9通路作用机制。[方法]使用线栓法制作大鼠大脑中动脉闭塞(MCAO)脑缺血模型,缺血90 min后行再灌注,治疗组予针刺干预,于再灌注6h、5d后将大鼠取材,进行荧光定量聚合酶链反应(RT-PCR)法检测,观察局灶性脑缺血模型大鼠脑组织TLR9、TNF-α、IRF7、IFN-β的m RNA表达及针刺的干预作用。[结果]针刺能明显增强神经保护因子IFN-βm RNA的表达(P<0.05)。TLR9信号通路m RNA的表达对比均无统计学差异(P>0.05)。[结论]本针刺法治疗脑梗死通过增强神经保护因子IFN-βm RNA的表达,改善脑缺血再灌注造成的神经功能活动障碍。本研究尚不能表明该针刺法能抑制TLR9信号通路m RNA的表达。[Objective]To observe the intervention effect of mRNA signaling pathway acupuncture therapy on cerebral ischemia reperfusion model of early TLR9,the infarct marginal zone TNF-α,IRF7,IFN-β;elucidating the mechanism of acupuncture treatment of cerebral infarction.[Methods]Use line plug method cerebral artery occlusion(MCAO)on rat cerebral ischemia model,ischemia reperfusion after 90 min,acupuncture group of rats received intervention of acupuncture method after reperfusion 6 h,5 d,the rats were decapitated,fluorescence quantitative polymerase chain reaction(RT-PCR)assay,to observe the odel of focal cerebral ischemia in rat brain TLR9,TNF-α,IRF7,IFN-βmRNA expression and intervention effect of acupuncture.[Results]This acupuncture method can obviously enhance the nerve protection factor IFN-βmRNA expression(P<0.05).[Conclusion]This acupuncture can treat of cerebral infarction by increasing nerve protection factor IFN-βmRNA expression,reducing the number of neurons injury,enhancing the capacity of brain tissue repair,and improving cerebral ischemia reperfusion injury of nerve function activities obstacles.This reserch cannot indicate the treatment could inhibition the mRNA expression of TLR9 pathway.

关 键 词：针刺 脑缺血再灌注 大鼠模型 TLR9 TNF-Α IRF7 IFN-Β mRNA 

分 类 号：R743[医药卫生—神经病学与精神病学]