胃癌高表达转录本1对胃癌细胞株AGS增殖、周期的影响及其机制  

Effect of gastric carcinoma highly expressed transcript 1 on proliferation and cell cycle in gastric cancer AGS cells

作　　者：万颖[1] 严芝强 韦四喜 夏英 刘娟娟[1] 周春欢 黄海[1,2] WAN Ying;YAN Zhiqiang;WEI Sixi;XIA Ying;LIU Juanjuan;ZHOU Chunhuan;HUANG Hai(Guizhou Medical University,Guiyang 550004,China)

机构地区：[1]贵州医科大学,贵阳550004 [2]贵州医科大学附属医院 [3]贵阳中医学院第一附属医院

出　　处：《山东医药》2018年第11期18-21,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81460364;81760429);贵阳市科技计划项目(20161001015)

摘　　要：目的观察胃癌高表达转录本1(GHET1)对胃癌细胞株AGS增殖、周期的影响,并探讨其机制。方法将干扰序列si-GHET1、阴性对照序列si-NC、空白对照序列转染进AGS细胞中(分别计为干扰组、阴性组、空白组),采用real-time PCR法检测各组细胞GHET1,CCK-8法检测细胞增殖能力,流式细胞术检测细胞周期,Western blotting法检测细胞周期相关蛋白。结果干扰组、阴性组、空白组GHET1相对表达量分别为0.42±0.06、1、1.15±0.19,干扰组与其余两组比较,P均<0.05;48 h细胞OD450值分别为0.57±0.04、0.93±0.05、1.03±0.06,72 h细胞OD450值分别为0.69±0.10、1.54±0.08、1.58±0.14,干扰组与其余两组比较,P均<0.05;G0/G1期细胞比例分别为62.01%±4.56%、47.03%±3.36%、44.65%±3.44%,S期细胞比例分别为20.34%±3.29%、32.25%±1.78%、32.96%±2.58%,干扰组与其余两组比较,P均<0.05;CDK2相对表达量分别为0.60±0.08、0.81±0.07、0.81±0.02,CDK4相对表达量分别为0.57±0.03、0.89±0.08、0.87±0.06,CDK6相对表达量分别为0.62±0.09、0.86±0.07、0.92±0.07,Cyclin D相对表达量分别为0.58±0.03、0.74±0.02、0.70±0.04,Cyclin E相对表达量分别为0.33±0.03、0.39±0.01、0.39±0.01,干扰组与其余两组比较,P均<0.05。结论 GHET1可促进AGS细胞增殖,缩短细胞周期,其机制可能通过上调细胞周期相关蛋白表达来实现。bjective To observe the effects of gastric carcinoma high expressed transcript 1(GHET1)on the proliferation and cell cycle of gastric cancer AGS cells.Methods Interference si-GHET1,negative control si-NC,blank control were transfected into AGS cells as the interference group,negative control group,and control group,respectively.The real-time PCR was used to examine the expression of GHET1 in each group.CCK-8 assay was performed to evaluate the cell proliferation ability.Cell cycle was measured by flow cytometry.Western blotting was used to analyze the expression of cell cycle-related proteins.Results In the interference group,negative control group,and control group,the expression of GHET1 was 0.42±0.06,1,and 1.15±0.19;the OD 450 values were 0.57±0.04,0.93±0.05,and 1.03±0.06 at 48 h,and 0.69±0.10,1.54±0.08,and 1.58±0.14 at 72 h;the ratio of G 0/G 1 phase was 62.01%±4.56%,47.03%±3.36%,and 44.65%±3.44%,and the ratio of S phase was 20.34%±3.29%,32.25%±1.78%,and 32.96%±2.58%;the relative protein expression of CDK2 was 0.60±0.08,0.81±0.07,and 0.81±0.02;the relative protein expression of CDK4 was 0.57±0.03,0.89±0.08,and 0.87±0.06;the relative protein expression of CDK6 was 0.62±0.09,0.86±0.07,and 0.92±0.07;the relative protein expression of Cyclin D was 0.58±0.03,0.74±0.02,and 0.70±0.04;the relative protein expression of Cyclin E was 0.33±0.03,0.39±0.01,and 0.39±0.01;significant differences were found in the above parameters between the interference group and the other groups(all P<0.05).Conclusion GHET1 may promote cell proliferation and shorten the cell cycle through up-regulating the cell cycle-related protein in AGS cells.

关 键 词：胃肿瘤 胃癌 长链非编码RNA 胃癌高表达转录本1 细胞增殖 细胞周期 细胞周期相关蛋白 

分 类 号：R735.2[医药卫生—肿瘤]