基于mt COI基因序列的DNA条形码技术在江苏沿江长角血蜱鉴定中的应用  

Application of DNA barcoding technolodge based on the mitochondrial gene COI to identify Haemaphysalis longicornis along the Yangtse River in Jiangsu province

作　　者：杨鹏飞[1] 燕清丽[1] 苏琦[1] 何芳 唐丽[1] 邢亚东[1] 刘纯成[1] 邵忠贤[1] 蔡蓉[1] 胡锦流[1] 姚海波[1] 何南江 YANG Peng-fei;YAN Qing-li;SU Qi;HE Fang;TANG Li;XING Ya-dong;LIU Chun-cheng;SHAO Zhong-xian;CAI Rong;HU Jing-liu;YAO Hai-bo;HE Nan-jiang(Huai an Municipal Center for Disease Control and Prevention,Jiangsu Huai an 223001,China)

机构地区：[1]淮安市疾病预防控制中心,江苏淮安223001

出　　处：《江苏预防医学》2018年第2期135-139,共5页Jiangsu Journal of Preventive Medicine

基　　金：淮安市科技项目(重点研发计划;HAS201616)

摘　　要：目的了解长角血蜱在盱眙地区(JS种群)的遗传多样性和不同地理种群间的分子进化关系,为该地区长角血蜱及其携带的病原体防控提供理论依据。方法采集盱眙县1例发热伴血小板减少综合征(SFTSV)确诊病例家附近的长角血蜱,并对其mt COI基因序列进行系统进化分析、种群遗传多样性分析及遗传结构分析。结果采集寄生蜱20只,游离蜱23只。JS种群的mt COI基因的核苷酸同源性为97.00%~100.00%,属"Compound"进化谱系枝。JS种群共有5个单倍型,单倍型多样性Hd=0.917,核苷酸多样性为π=0.005,多态性位点S=9。Tajima's D值为-1.456,核苷酸错配分布图为单峰。结论江苏沿江地区长角血蜱JS种群可能经历种群的扩张。Objective To determine the genetic diversity of Haemaphysalis longicornis(H.longicornis)in the area of Xuyi county(JS population)and molecular evolutional relation with other populations;to provide basis for the prevention and control of H.longicornis and tick-borne diseases.Methods Ticks in the vicinity of 1 case of fever accompanied by thrombocytopenia syndrome(SFTSV)in Xuyi county were collected.The phyogenetic relationship among populations,the genetic diversity of populations and the population genetic structure were used for analyzing the sequences of the mt COI of H.longicornis.Results A total of 20 parasitic ticks and 23 free ticks were collected.The mt COI gene nucleotide homology of JS population was from 97.00%to 100.00%,belonging to the"Compound"phylogenetic branch.There were 5 haplotypes in the JS population,the haplotype diversity Hd was 0.917,the nucleotide diversityπwas 0.005,the polymorphism site S was 9,the Tajima s D value was-1.456.The mismatch distribution pattern of population JS was single-peak.Conclusion The JS population of H.longicornis along the Yangtse River might go through population expansion.

关 键 词：长角血蜱 系统进化树 种群遗传多样性 种群遗传结构 

分 类 号：R117[医药卫生—公共卫生与预防医学]