ApoE基因多态性与脑电图慢波的关联性研究  

Association between ApoE gene polymorphism and slow waves of electroencephalogram

作　　者：罗伏钢[1] 李静 陈梅芳[1] 迟淑梅 宋明芬[2] LUO Fugang;LI Jing;CHEN Meifang(Department of Geriatrics,Hangzhou Seventh People's Hospital,Hangzhou 310013,China)

机构地区：[1]杭州市第七人民医院老年科,310013 [2]杭州市第七人民医院分子生物学实验室,310013 [3]杭州市第七人民医院神经内科,310013

出　　处：《浙江医学》2018年第6期564-567,共4页Zhejiang Medical Journal

基　　金：国家自然科学基金(81601183);杭州市科技发展计划项目重点专科专病科研攻关专项(20150633B10;20160533B28)

摘　　要：目的研究ApoE基因多态性在脑电图慢波患者中的分布及其对慢波始发年龄的影响。方法简易智能量表(MMSE)>24分且无脑梗死史者进行脑电图检查,出现慢波的患者进入研究组,同时收集健康对照,采集每例研究对象的基本信息与血液,使用PCR法测定Apo E基因多态性。采用χ~2检验比较研究组和对照组之间各基因型的分布差异,使用方差分析比较研究组各基因型间脑电图慢波出现年龄的差异。结果研究组ε4基因型频率51.78%(58/112)和ε4等位基因频率36.16%(81/224)明显高于对照组的16.08%(18/112)和10.71%(24/224)(均P<0.05)。研究组ε2和ε3基因型频率11.61%(13/112)和36.61%(41/112)及其等位基因频率9.38%(21/224)和54.46%(122/224)显著低于相应的对照组(均P<0.05)。研究组Apo Eε4基因型的脑电图慢波出现年龄[(64.07±5.57)岁]明显小于ε2[(70.69±8.29)岁]和ε3型[(67.73±6.10)岁](均P<0.05)。结论 Apo Eε4基因型与脑电图慢波相关,且该基因型慢波始发年龄有年轻化的趋势。Objective To study the distribution of ApoE gene polymorphism and its relation to slow waves of electroencephalogram.Methods Patients with Mini Mental State Examination(MMSE)>24 and no cerebral infarction history were examined by electroencephalogram.Those with slow waves in electroencephalogram were recruited in the research group and the healthy controls were also recruited.The ApoE gene polymorphism was determined by PCR method in two group.The association of ApoE gene polymorphism with the onset age of slow waves in research group was analyzed.Results The research group had significantly higherε4 genotype frequency(51.78%,58/112)andε4 allele frequency(36.16%,81/224)as compared to those of the control group(16.08%,18/112 and 10.71%,24/224)(both P=0.000).ApoEε2 andε3 genotype frequency(11.61%,13/112 and 36.61%,41/112)and allele frequency(9.38%,21/224 and 54.46%,122/224)were significant lower in the research group as compared with those in the control group(P=0.02,0.000,0.002 and 0.001).Patients withε4 genotype had a significantly younger onset age of slow waves(64.07±5.57 years)as compared toε2 genotype(70.69±8.29 years)(P=0.000)andε3 genotype(67.73±6.10 years)(P=0.003).Conclusion ApoEε4 genotype is associated with slow waves of electroencephalogramand it is also related to early onset of slow waves than other genotypes.

关 键 词：载脂蛋白E基因多态性 脑电图 慢波 始发年龄 

分 类 号：R741.044[医药卫生—神经病学与精神病学]