甘肃黑猪H-FABP基因克隆、SNPs筛查及生物信息学分析  

H-FABP gene cloning,SNPs screening and bioinformatics analysis in Gansu black pig

作　　者：农伟伦 毕英杰[1] 李宏旭 张丽[1] NONG Weilun;BI Yingjie;LI Hongxu;ZHANG Li(College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730124,China)

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730124

出　　处：《浙江农业学报》2018年第3期378-385,共8页Acta Agriculturae Zhejiangensis

基　　金：西北民族大学国家级大学生创新创业训练计划(201710742094)

摘　　要：采用生物信息学方法从分子水平对甘肃黑猪H-FABP基因编码蛋白质的功能和性质进行分析。采用DNA池和直接测序技术对甘肃黑猪H-FABP基因进行SNPs快速筛查及其编码区蛋白质功能预测。结果表明,甘肃黑猪H-FABP基因编码区全长402 bp,编码133个氨基酸残基,共检测出4个SNPs,分别为C^(734)T-Intron1、T^(152)C-Exon2、G^(30)A-Intron2、T^(121)G-Intron2,其中T^(152)C-Exon2为错义突变,氨基酸由原来的异亮氨酸(Ile)转变为苏氨酸(Thr)。生物信息学预测发现,甘肃黑猪H-FABP蛋白是一种稳定的亲水蛋白,蛋白质二三级结构均以β-折叠为主的混合型蛋白。This study uses bioinformatics method to analyze the function and property of H-FABP gene encoding protein of Gansu black pig.DNA pooling and direct sequencing techniques were used for SNPs rapid screening and the function prediction of H-FABP protein of Gansu black pig.The results showed that the CDS region of H-FABP gene in Gansu black pig was 402 bp,encoding 133 amino residues,4 SNPs in CDS region including C 734 T-Intron1,T 152 C-Exon2,G 30 A-Intron2 and T 121 G-Intron2.Among them,T 152 C-Exon2 was a missense mutation,and the amino acids were converted from threonine(Ile)to threonine(Thr).The bioinformatics prediction showed that H-FABP protein of Gansu black pig was a stable hydrophilic protein,and the secondary structure and tertiary structure were mixed protein mainly withβ-fold.

关 键 词：甘肃黑猪 H-FABP基因 SNPS 等位基因频率 生物信息学 

分 类 号：S828[农业科学—畜牧学]