高压冷冻和冷冻替代技术制备的酵母细胞超微结构  

Study on the ultrastructure of yeast cells by high pressure freezing and freezing substitution

作　　者：冯凤萍 栾会芹 冯红丽 郭振玺[4] 张建设 FENG Feng-ping;LUAN Hui-qin;FENG Hong-li;GUO Zhen-xi;ZHANG Jian-she(Marine Science College of Zhejiang Ocean University,National Engineering Research Center of Marine Facilities Aquaculture,Zhoushan Zhejiang 316022;Beijing Key Laboratory of Rehabilitation Technical Aids for Old.Age Disability,Key Laboratory of Intelligent Control and Rehabilitation Technology of the Ministry of Civil Affairs,National Research Center for Rehabilitation Technical Aids,Beijing 100176;National Laboratory of Biomacromolecules,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101;Center for Biological Imaging,Core Facility for Protein Research,　　Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]国家海洋设施养殖工程技术研究中心,浙江海洋大学海洋科学与技术学院,浙江舟山316022 [2]北京市老年功能障碍康复辅助技术重点实验室,民政部智能控制与康复技术重点实验室,国家康复辅具研究中心,北京100176 [3]生物大分子国家重点实验室,中国科学院生物物理研究所,北京100101 [4]生物成像中心蛋白质科学研究平台,中国科学院生物物理研究所,北京100101

出　　处：《电子显微学报》2018年第2期195-200,共6页Journal of Chinese Electron Microscopy Society

基　　金：海洋科学浙江省重中之重学科开放课题(No.20160106);中央级公益性科研院所基本科研业务费专项资金项目(No.118009001000160001).

摘　　要：高压快速冷冻技术可以使生物样品快速冷冻固定,其组织、细胞结构接近于生理状态,有效减少了样品制备过程中化学试剂对细胞结构的破坏,并能够观察到细胞器近似自然状态下的超微结构。本文应用高压冷冻和冷冻替代技术进行酵母细胞的电镜样品制备,观察到了清晰的细胞壁、细胞核、液泡、线粒体及内质网等亚细胞器的超微结构,为酵母细胞的电镜样品制备提供了更多的方法和手段。High pressure freezing(HPF)can preserve excellent cellular structures in a near-native state with samples in glasslike amorphous ice quickly.Next freezing substitution(FS)steps are processed at low temperatures.We applied HPF and FS technology on yeast Saccharomyces cerevisiae and observed the ultrastructure.The goal of this study is to provide some technological references for researchers that want to investigate at the ultrastructural level in yeasts.

关 键 词：高压冷冻 冷冻替代 酵母 超微结构 

分 类 号：Q942[生物学—植物学] Q949.326.1