S-腺苷甲硫氨酸对血管平滑肌细胞PDGFmRNA表达的影响  

Effect of S-adenosylmethionine on the Expressions of PDGF mRNA of Vascular Smooth Muscle Cells

作　　者：金成吉[1] 段薇[1] 曲建梅[1] 彭勃[1] 刘璟瑶[1] 孙建平[1] JIN Cheng-ji;DUAN Wei;QU Jian-mei;PENG Bo;LIU Jing-yao;SUN Jian-ping(Department of Endocrinology,Affiliated Zhongshan Hospital Dalian University,Dalian,Liaoning Province,116001 China)

机构地区：[1]大连大学附属中山医院内分泌内科,辽宁大连116001

出　　处：《系统医学》2017年第22期15-17,共3页Systems Medicine

摘　　要：目的观察S-腺苷甲硫氨酸对血小板源性生长因子m RNA表达及对血管平滑肌细胞(VSMCs)增殖与迁移的影响。方法于2015年3月—2016年3月在大连大学附属中山医院实验中心将8周龄SD大鼠30只分为空白对照组10只、对照组10只和SAMe组10只。造模后SAMe组腹腔内注射SAMe[15 mg/(kg·d)],对照组注射相同剂量的生理盐水,观察大鼠正常颈动脉内膜损伤前后VSMCs基因甲基化差异、颈动脉管腔面积及内膜/中膜面积比值、测定VSMCs增殖、各组细胞PDGF m RNA表达水平。结果大鼠颈动脉内膜损伤后VSMCs的PDGF启动子去甲基化。对照组和SAMe组VSMCs迁移距离分别为(645±143)m和(468±87)m;对照组和SAMe组VSMCs 490nm吸光度A值分别为(2.47±0.64)nm和(1.92±0.52)nm;PDGF m RNA表达的相对比值(PDGF/GAPDH),对照组和SAMe组分别为(1.63±0.52)和(0.64±0.20);对照组和SAMe治疗组大鼠颈动脉内膜面积分别(0.468±0.092)mm2和(0.275±0.064)mm2,管腔面积分别为(0.437±0.087),(0.635±0.104)mm2,SAMe组管腔面积大于对照组(P<0.01)。两组情况比较,差异有统计学意义(P<0.05)。结论大鼠颈动脉内膜损伤引起VSMCs中PDGF启动子去甲基化,SAMe抑制VSMCs中PDGF m RNA表达和VSMCs增殖和迁移。Objective To observe the effect of S-adenosylmethionine on the expressions of PDGF mRNA of vascular smooth muscle cells.Methods 30 cases of SD rats in the experimental center in our hospital from March 2015 to March 2016 were divided into the blank control group,control group and SAMe group with 10 cases in each,after molding,the rats in SAMe adopted the intraperitoneal injection of SAMe each day[15 mg/(kg·d)],the control group were injected with the same dosage of normal saline,and the difference in the VSMCs methylation before and after the normal carotid artery intima injury,carotid lumen area,ratio of intima/middle membrane area were observed and the VSMCs proliferation and the expression levels of PDGF mRNA of each group were measured.Results The migration distances of VSMCs in the control group and SAMe group were respectively(645±143)m and(468±87)m,and the VSMCs 490 nm absorbance A values in the control group and in the SAMe group were respectively(2.47±0.64)nm and(1.92±0.52)nm,and the relative ratio of PDGF mRNA expression(PDGF/GAPDH)in the control group and in the SAMe group was respectively(1.63±0.52)and(0.64±0.20),and the intima area of carotid artery and lumen area of rats in the control group and in the SAMe group were respectively(0.468±0.092)mm2,(0.275±0.064)mm2 and(0.437±0.087),(0.635±0.104)mm2,and the lumen area in the SAMe group was higher than that in the normal saline injection group(P<0.01),and the difference was statistically significant(P<0.05).Conclusion The PDGF promoter demethylation of VSMCs is caused by the carotid intima injury of rats,and the SAMe controls the expression of PDGF mRNA and proliferation and migration of VSMCs.

关 键 词：S-腺苷甲硫氨酸 血管平滑肌细胞 甲基化 血小板源性生长因子 

分 类 号：R364[医药卫生—病理学]