柚皮素对人肺鳞癌NCI-H2170细胞增殖、凋亡的影响  

Effects of Naringenin on the proliferation and apoptosis of lung cancer cell NCI-H2170

作　　者：曾德贵[1] 陈保安 吴尚 黄慧 伍俊 ZENG Degui;CHEN Baoan;WU Shang;HUANG Hui;WU jun(Department of Pharmacy,Shenzhen Center for Chronic Disease Prevention and Control,Guangdong Province,Shenzhen 518020,China;Institute of Respiratory Diseases,Guangdong Medical University,Guangdong Province,Zhanjiang 524023,China;Department of Biochemistry and Molecular Biology,Guangdong Medical University,Zhanjiang,Guangdong 524023,China)

机构地区：[1]广东省深圳市慢性病防治中心药剂科,广东深圳518020 [2]广东医科大学呼吸疾病研究所,广东湛江524023 [3]广东医科大学生物化学与分子生物学教研室,广东湛江524023

出　　处：《中国医药导报》2018年第8期4-8,共5页China Medical Herald

基　　金：广东省社会发展领域科技计划项目(2014A020212292);广东省深圳市科技计划项目(201202105)

摘　　要：目的探讨柚皮素对人肺鳞癌细胞NCI-H2170的增殖、凋亡及细胞周期的影响。方法用MTT法检测柚皮素对NCI-H2170细胞增殖的影响,Hoechst33258/PI染色检测NCI-H2170细胞形态学变化,流式细胞术(FCM)检测细胞周期的改变。Western blot检测柚皮素处理NCI-H2170细胞24 h后PARP、Bcl-2、Bid、procaspase-3及procaspase-8蛋白的表达。结果柚皮素处理人肺鳞癌NCI-H2170细胞24 h后,浓度依赖性地抑制NCI-H2170细胞增殖;其中12.5μmol/L处理组与对照组比较,差异无统计学(P>0.05);25、50、100μmol/L处理组与对照组比较,差异有统计学意义(P<0.05)。流式细胞仪分析结果显示:不同浓度的柚皮素处理NCI-H2170细胞24 h后,与对照组比较,12.5μmol/L处理组差异无统计学意义(P>0.05);25、50、100μmol/L处理组G1期细胞显著增多,S期及G2期细胞显著减少,差异均有统计学意义(P<0.05)。25、50、100μmol/L处理组与对照组相比,柚皮素上调Bid表达,促进PARP降解,Bcl-2、procaspase-3和procaspase-8降低,差异均有统计学意义(P<0.05)。结论柚皮素抑制人肺鳞癌NCI-H2170细胞增殖,并能诱导NCI-H2170细胞产生G1期阻滞,诱导细胞凋亡。Objective To investigate the effects of Naringenin on the proliferation, apoptosis and cell cycle of human lung squamous cell carcinoma cell line NCI-H2170. Methods The proliferation of NCI-H2170 cells was detected by MTT assay. The morphological changes of NCI-H2170 cells were detected by Hoechst33258/PI staining. Cell cycle changes were measured by flow cytometry(FCM). Western blot was used to detect the expression of PARP, Bid, Bcl-2,procaspase-3 and procaspase-8 in NCI-H2170 cells treated with naringenin. Results The proliferation of human lung squamous cell carcinoma NCI-H2170 cells were inhibited in a dose-dependent manner after treating with Naringenin for 24 h. It was not significant difference between 12.5 μmol/L treatment group and the control group(P > 0.05).However,25, 50, 100 μmol/L treatment groups were significantly different comparing to the control group(P < 0.05). The results of flow cytometry analysis showed that it was not significant difference between 12.5 μmol/L treatment group and the control group(P > 0.05). However, after NCI-H2170 cells were treated with 25, 50, 100 μmol/L naringenins, the number of cells had significantly increased in G_1 phase, desceased in S phase and G_2 phase, comparing to the control group(P < 0.05). Naringenin up-regulated Bid expression, promoted the degradation of PARP and the decrease of Bcl-2, proaspase-3 and proaspase-8 in NCI-H2170 cells,comparing to the control group(P < 0.05). Conclusion Naringenin inhibits the proliferation of human lung squamous cell carcinoma cell line NCI-H2170 and induces the G_1 pha se arrest in NCI-H2170 cells and induces cell apoptosis.

关 键 词：柚皮素 人肺鳞癌NCI-H2170细胞 细胞凋亡 增殖 细胞周期 

分 类 号：R734.2[医药卫生—肿瘤]