瑞香狼毒根部烷烃类提取物GC-MS分析及抑瘤活性初探  

Chemical Components and Antitumor Activity of Root Extract of Stellera chamaejasme L.

作　　者：赵琳 杨喜花[2] 杨永明[2] 李树萍[1] 王卉 王跃凤 张生万[1] ZHAO Lin;YANG Xi-hua;YANG Yong-ming;LI Shu-ping;WANG Hui;WANG Yue-feng;ZHANG Sheng-wan(Shanxi University,College of Life Sciences,Taiyuan 030006,China;Shanxi Cancer Institute,Department of comparative medicine,Taiyuan 030013,China)

机构地区：[1]山西大学生命科学学院,太原030006 [2]山西省肿瘤研究所比较医学部,太原030013

出　　处：《天然产物研究与开发》2018年第4期621-628,共8页Natural Product Research and Development

基　　金：山西省实验动物专项(2015k09)

摘　　要：通过正己烷提取瑞香狼毒根部成分,采用气相色谱-质谱联用技术对瑞香狼毒根部烷烃类提取物(SRH)的成分进行分析,同时考察SRH对H22皮下荷瘤小鼠的抑瘤作用。结果表明:通过GC-MS分析,SRH共分离得到55个组分,结构确定的有44个,占总峰面积的97.73%,其中烃类化合物11个,芳香族化合物10个,酸类化合物有9个,酯类化合物有7个,醇类化合物有4个,其他类型化合物有3个。其中含量在1%以上的物质有(Z,Z)-9,12-十八烷二烯酸,邻苯二甲酸-2-乙基己酯,9,17-十八烷二烯,反式角鲨烯,γ-谷甾醇,邻苯二甲酸二丁酯,n-十六酸,硬脂酸,二十酸,菜油甾醇,占总峰面积90.74%,检测出的角鲨烯,亚油酸,菜油甾醇都具有一定的抗肿瘤活性,在瑞香狼毒抗肿瘤方面的研究未见报道;同时SRH在实验前期对肿瘤的生长起到了很好的抑制作用,尤其是SRH低剂量,其抑制率达到21.5%。该结果为瑞香狼毒根部成分的物质分析及抑瘤应用前景提供了一定的理论依据。The components of the SRH which using hexane extracts the root of Stellera chamaejasme L.were analyzed by Gas Chromatography-Mass Spectrometry.Meanwhile,study the effects on H 22 cancer mice.The results indicated that 44 structures of the root of Stellera chamaejasme L.were identified by GC-MS analysis,these included 12 kinds of hydrocarbon compounds,10 kinds of aromatic compounds,9 kinds of acids compounds,7 kinds of esters compounds,4 kinds of alcohols compounds and 2 other types of compounds.The contents above 1%of the root of Stellera chamaejasme L.were(Z,Z)-9,12-octadecadienoic acid,di-2-ethylhexyl phthalate,9,17-octadecadienal,triacontahexaene,.gamma.-sitosterol,dibutyl phthalate,n-hexadecanoic acid,octadecanoic acid,eicosanoic acid,whose total peak area is 90.74%,detection of squalene,linoleic acid,campesteral have certain antitumor activity ani-tumor which has not been reported in Stellera chamaejasme L.The SRH was effective in inhibiting the growth of the tumor;especially the low dose of SRH,the inhibition rate reached 21.5%.The results provide a theoretical basis for the analysis ofmaterial composition and anti-tumor effects about the roots of Stellerachamaejasme L.

关 键 词：瑞香狼毒根部 烷烃类提取物 气相色谱-质谱法(GC-MS) 正己烷 抑瘤 

分 类 号：R284.1[医药卫生—中药学]