利拉鲁肽对神经母细胞瘤细胞牵张损伤的神经保护作用  

The neuroprotective effect of liraglutide on stretch injury of SH-SY5Y cells

作　　者：李建鑫 朱晓龙 苏景良 李建伟 梁海乾 孙洪涛 Li Jianxin;Zhu Xiaolong;Su Jingliang;Li Jianwei;Liang Haiqian;Sun Hongtao(Department of Neurology,Affiliated Hospital of Logistics University of PAP,300162 Tianjin,China)

机构地区：[1]中国人民武装警察部队后勤学院附属医院脑科中心,天津300162

出　　处：《中国临床保健杂志》2018年第2期229-233,共5页Chinese Journal of Clinical Healthcare

基　　金：国家自然科学基金面上项目(81471275)

摘　　要：目的探讨胰高血糖素样肽-1类似物利拉鲁肽对神经母细胞瘤细胞(SH-SY5Y)牵张损伤的神经保护作用。方法本实验以SH-SY5Y细胞作为研究对象,首先采用MTT实验筛选最适宜的利拉鲁肽处理浓度,然后利用可控性细胞损伤装置(CIC-II)建立细胞牵张损伤模型。将实验分为3组,即对照组、细胞牵张损伤组、利拉鲁肽治疗组(1μM利拉鲁肽处理SH-SY5Y细胞24 h)。乳酸脱氢酶(LDH)法检测各组细胞毒性;蛋白免疫印迹实验检测凋亡相关蛋白Bax、抗凋亡蛋白Bcl-2的表达和Caspase-3的表达水平。结果 MTT实验显示,利拉鲁肽处理SH-SY5Y细胞适宜的保护性浓度为1μM。LDH实验显示,与对照组相比,牵张损伤组LDH漏出量显著增多;与细胞损伤组相比,利拉鲁肽治疗组细胞LDH漏出量明显降低(P<0.05);蛋白免疫印迹实验显示:与对照组相比,细胞损伤组Bax表达水平显著增强,Bcl-2表达显著减少,Caspase-3表达明显增高,细胞凋亡百分比显著增高;与细胞损伤组相比,利拉鲁肽治疗组Bax和Caspase-3蛋白表达水平降低(P<0.05),Bcl-2表达显著增多(P<0.01),细胞凋亡百分比显著降低。结论利拉鲁肽可以减轻细胞损伤引起的LDH释放水平,利拉鲁肽能显著降低损伤细胞的凋亡。利拉鲁肽通过抑制细胞凋亡进而发挥其神经保护作用。Objective To investigate the neuroprotective effect of glucagon-like 1 analogue liraglutide on neuroblastoma cells(SH-SY5Y).Methods SH-SY5Y cells were selected as the research object and the most appropriate concentration of liraglutide treatment was choosed by MTT assay.Then cell stretch injury model was established using cell injury controlled II(CIC-II).The experiment cells were divided into three groups:control,cell injury and liraglutide treated groups(1μM liraglutide treating SH-SY5Y cells for 24 h).Lactate dehydrogenase(LDH)was used to detect cell toxicity.The expression of Bax,Bcl-2 and caspase-3 were detected by Western blotting.Results The MTT assay showed that the protective concentration of liraglutide in SH-SY5Y cells was 1μM.Compared with the control group,the leakage of LDH in stretch injury group was significantly increased,and the leakage of LDH inliraglutide group was significantly decreased than that in the control group(P<0.05).Western blotting results showed that the expression of Bax and caspase-3 were significantly increased compared with the control group,but the expression of Bcl-2 was significantly decreased,and the percentage of cell apoptosis was significantly increased.Compared with the cell injury group,the expression of Bax and caspase-3 were decreased ofliraglutidegroup(P<0.05),but the expression of Bcl-2 was significantly increased,and the percentage of apoptosis was decreased(P<0.01).Conclusion Liraglutide can reduce the LDH release induced by cell injury and the apoptosis of injured cells.thus it is speculated that liraglutide should exert its neuroprotective effect by inhibiting cell apoptosis.

关 键 词：神经母细胞瘤 神经保护药 细胞凋亡 细胞增殖 

分 类 号：R739.4[医药卫生—肿瘤]