BaP和Cd单一复合对BaP蚯蚓亚细胞分配的影响  

The influence of the single benzo[a]pyrene pollution or combined pollution with cadmium on the distribution and accumulation of benzo[a]pyrene in subcellular fractions of Eisenia fetida

作　　者：周丽娜[1,2,3] 周静 刘潇雅[1] 常大丽 李辉信 胡锋[1] 徐莉[1,2] ZHOU Li-na;ZHOU Jing;LIU Xiao-ya;CHANG Da-li;LI Hui-xin;HU Feng;XU Li(College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095,China;Jiangsu Organic Solid Waste Resource Cooperative Innovation Center,Nanjing Agricultural University,Nanjing 210095,China;Zhejiang Renxin Testing Research Institute Co.,Ltd.,Ningbo 315000,China)

机构地区：[1]南京农业大学,资源与环境科学学院,江苏南京210095 [2]南京农业大学,江苏省有机固体废弃物资源化协同创新中心,江苏南京210095 [3]浙江人欣检测研究院股份有限公司,浙江宁波315000

出　　处：《中国环境科学》2018年第4期1510-1516,共7页China Environmental Science

基　　金：国家自然科学基金资助项目(41371469);国家重点研发计划资助项目(2017YFD0800305);公益性行业(农业)科研专项经费资助项目(201503121);江苏省优势学科资助项目.

摘　　要：选用钙离子通道阻断剂氯化镧(La Cl3)和巯基蛋白阻断剂N-乙马来酰亚胺(NEM)对赤子爱胜蚓进行预暴露,然后构建Ba P单一或Cd-Ba P复合污染,研究不同阻断剂对Ba P在蚯蚓不同亚细胞组分(Fraction C:细胞溶质组分;Fraction D:固体颗粒组分;Fraction E:细胞碎片组分)中分配积累特征的影响.结果表明,无论单一或复合污染,Ba P主要分布于细胞碎片组分中(占55.42%~69.96%),其次为固体颗粒组分(占27.91%~32.90%),在细胞溶质组分中的浓度最低(占2.13%~11.67%).单一Ba P污染下,两种阻断剂对Ba P的作用相近,即La Cl3和NEM的加入均能不同程度地促进3个亚细胞组分中Ba P的积累.而在Cd和Ba P复合污染下,两种阻断剂对Ba P的分布积累的影响略有不同,La Cl3能够促进复合污染下Ba P在3个亚细胞组分中的积累,而NEM促进了复合污染下Ba P在固体颗粒组分和细胞碎片组分中的积累,但抑制了Ba P在细胞溶质组分中的积累,浓度从原来的0.99mg/kg降低至0.59mg/kg.因此,钙离子通道和巯基蛋白可能参与Ba P在蚯蚓亚细胞的分配积累,相比单一污染,Cd的复合污会进一步改变Ba P的积累分配特征.The influence of the calcium ion channel blocker(LaCl3)or the SH-blocker(Nitrogen-ethylmaleimide,NEM)on the distribution and accumulation patterns of the Benzoapyrene(BaP)in the three subcellular fractions of earthworm Eisenia fetida(Fraction C:associated with the cytosol;Fractions D:associated with granules;and Fraction E:associated with tissue fragments and cell membranes)were explored under the condition of single BaP contamination and the combined contamination of the BaP and cadmium(Cd).The results showed that BaP was mainly accumulated in the Fraction E(about 55.42%~69.96%of the total content)the Fraction D(about 27.91%~32.90%of the total content)and Fraction C(about 2.13%~11.67%of the total content)no matter under single or combined pollution.Under the single pollution of BaP,the addition of LaCl3and NEM could stimulate the BaP accumulation in three above subcellular fractions.While under the combined pollution,the effects were slightly different.LaCl3 could stimulated the BaP accumulation in three above subcellular fractions,but NEM inhibited the BaP accumulation in the Fraction C(from 0.99mg/kg to 0.59mg/kg),and enhanced the BaP accumulation in the Fraction D and E.Therefore,calcium ion channel and the sulfhydryl protein took participate in the BaP distribution in earthworm,and Cd addition changed the BaP distribution compared with the single BaP contamination.

关 键 词：苯并(A)芘 镉 赤子爱胜蚓(Eiseniafetida) 亚细胞组分 阻断剂 单一污染 复合污染 

分 类 号：X174[环境科学与工程—环境科学]