雌激素通过调控RCC2参与乳腺癌细胞凋亡  

Estrogen participates in the apoptosis of breast cancer cells by regulating RCC2

作　　者：王伟琦 郑亚冰 李昌[3] 刘春燕 汤俊怡 常晓天 

机构地区：[1]山东大学临床医学院,济南250012 [2]山东大学附属千佛山医院医学研究中心,济南250014 [3]滕州市中心人民医院病理科,滕州277500 [4]不详

出　　处：《新医学》2018年第4期248-254,共7页Journal of New Medicine

基　　金：山东省重大科技创新工程(2017CXGC1202);山东省重点研发计划(GG201703080038)

摘　　要：目的探索雌激素刺激乳腺癌病变过程中染色体缩合调控子2(RCC2)的作用及机制。方法收集乳腺标本,用蛋白免疫印迹法检测RCC2蛋白表达水平;分别用雌激素(雌二醇)、RCC2小干扰RNA(siRNA),雌激素联合RCC2 siRNA处理MCF-7细胞,检测细胞增殖与凋亡。结果与乳腺纤维瘤组织相比,RCC2在雌激素受体阳性(ER+)乳腺癌组织中的表达水平升高(P=0.001),在ER-乳腺癌组织中无升高(P=0.404)。抑制RCC2后ER+乳腺癌细胞系MCF-7增殖无变化(F_(处理)=0.003,P=0.957),但凋亡比例比Allstars siRNA组高(t=2.679,P=0.037);雌二醇处理MCF-7后增殖能力增强(F=110.323,P<0.001),细胞凋亡比例减弱(t=5.881,P=0.004);雌二醇与RCC2siRNA的交互效应在MCF-7细胞增殖方面无统计学意义(F=0.281,P=0.604),在细胞凋亡方面有统计学意义(F=18.897,P=0.002)。结论雌激素可能通过调控RCC2来抑制细胞凋亡,从而促进ER+乳腺癌癌变进程。Objective To explore the role of chromosomal condensation regulator 2(RCC2)during the process of estrogen promoting the progression of breast cancer.Methods Human breast tissues were collected.Western blot was utilized to quantitatively detect the expression level of RCC2 protein.MCF-7 cells were treated with estrogen or RCC2 siRNA and combined use of estrogen and RCC2 siRNA.The cell apoptosis and cell proliferation were detected.Results Compared with the breast fibroma tissues,the expression level of RCC2 in the ER+breast cancer tissues was significantly up-regulated(P=0.001),but there was no significant change in the ER-breast cancer tissues(P=0.404).After inhibiting the expression of RCC2,the proliferation of MCF-7 cells did not significantly change(F=0.003,P=0.957),whereas the apoptosis ratio of MCF-7 cells was significantly higher than that in the Allstars siRNA group(t=2.679,P=0.037).After treated with E2,the proliferation of MCF-7 cells was evidently enhanced(F=110.323,P<0.001),whereas the apoptosis ratio was considerably reduced(t=5.881,P=0.004).The interaction effect between estrogen and RCC2 siRNA had no statistical significance in the proliferation of MCF-7 cells(F=0.281,P=0.604),whereas yielded statistical significance in terms of cell apoptosis(F=18.897,P=0.002).Conclusion Estrogen inhibits cell apoptosis probably by regulating RCC2,thereby promoting the progression of ER+breast cancer.

关 键 词：染色体缩合调控子2 雌激素 乳腺癌 细胞凋亡 细胞增殖 

分 类 号：R737.9[医药卫生—肿瘤]