肾透明细胞癌中SFRP1、SFRP2基因启动子区甲基化状态的检测及其临床意义  

Detection and clinical significance of methylation in the promoter region of SFRP1 and SFRP2 genes in renal clear cell carcinoma

作　　者：殷凤朝[1] 李芳[2] 刘丽辉[1] 曹赫然[1] 李珅[1] 孙超[1] YIN Feng-chao;LI Fang;LIU Li-hui;CAO He-ran;LI Shen;SUN Chao(Department of Urology,the First Hospital of Shijiazhuang,Hebei Province,Shijiazhuang 050011,China;Department of Pathology,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China)

机构地区：[1]河北省石家庄市第一医院泌尿外科,河北石家庄050011 [2]河北医科大学第四医院病理科,河北石家庄050011

出　　处：《河北医科大学学报》2018年第3期267-271,共5页Journal of Hebei Medical University

基　　金：河北省医学科学研究重点课题(20160798)

摘　　要：目的探讨分泌型卷曲相关蛋白1(secreted frizzled-related protein 1,SFRP1)及分泌型卷曲相关蛋白2(secreted frizzled-related protein 2,SFRP2)基因启动子区甲基化状态与肾透明细胞癌(renal clear cell carcinoma,RCCC)的关系。方法采用甲基化特异性PCR(methylation specific PCR,MSP)法检测RCCC及其癌旁组织中SFRP1和SFRP2基因的甲基化情况;采用免疫组织化学SP法检测Wnt通路中心因子β-catenin蛋白表达。结果122例RCCC组织SFRP1基因和SFRP2基因甲基化发生率显著高于癌旁组织(P<0.05)。临床分期Ⅳ期RCCC组织SFRP1基因的甲基化发生率高于临床分期Ⅰ、Ⅱ、Ⅲ期(P<0.05)。RCCC组织中β-catenin蛋白的表达率明显高于癌旁组织(P<0.05)。高甲基化SFRP1基因在RCCC组织中和高甲基化SFRP2基因在RCCC组织中β-catenin蛋白的表达率明显高于未甲基化的RCCC组织(P<0.05)。结论 SFRP1、SFRP2基因甲基化可能参与由Wnt/β-catenin信号转导通路介导的RCCC的发生;SFRP1基因高甲基化可能与RCCC的侵袭及转移相关。Objective To investigate the promoter methylation status of secreted frizzled-related protein 1(SFRP1)and SFRP2 gene in renal clear cell carcinoma(RCCC).Methods MSP was used to examine the methylation status of SFRP1 and SFRP2 gene in RCCC tissues and corresponding adjacent non-cancerous tissues.The expression ofβ-catenin in Wnt pathway was detected by immunohistochemical streptavidin/peroxidase(SP)method.Results Methylation frequencies of SFRP1 and SFRP2 gene in 122 cases of RCCC were significantly higher than that in corresponding adjacent non-cancerous tissues(P<0.05).The incidence of methylation of SFRP1 gene in stageⅣwas significantly higher than that in stageⅠ,ⅡandⅢin RCCC tissues(P<0.05).The expression ofβ-catenin in RCCC tissues was significantly higher than that in corresponding adjacent non-cancerous tissues(P<0.05).The expression ofβ-catenin in hypermethylated SFRP1 and SFRP2 gene in RCCC tissues was significantly higher than that in unmethylated RCCC tissues(P<0.05).Conclusion SFRP1,SFRP2 gene methylation may be involved in the occurrence of RCCC mediated by Wnt/β-catenin signal transduction pathway,and high methylation of SFRP1 gene was associated with invasion and metastasis of RCCC.

关 键 词：肾肿瘤 分泌型卷曲相关蛋白 甲基化 

分 类 号：R737.11[医药卫生—肿瘤]