促红细胞生成素对大鼠蛛网膜下腔出血后血管痉挛的防治作用  

Preventive and therapeutic effects of erythropoietin on cerebral vasospasm after subarachnoid hemorrhage in rats

作　　者：熊平[1] 唐晓平[1] XIONG Ping;TANG Xiao-ping(Department of Neurosurgery,Affiliated Hospital,North Sichuan Medical College,Nanchong 637000,China)

机构地区：[1]川北医学院附属医院神经外科,四川南充637000

出　　处：《中国临床神经外科杂志》2018年第4期254-257,共4页Chinese Journal of Clinical Neurosurgery

摘　　要：目的探讨促红细胞生成素(EPO)对大鼠蛛网膜下腔出血(SAH)后脑血管痉挛(CVS)的影响及其机制。方法将60只成年SD大鼠随机分为正常组(6只)、假手术组(18只)、SAH组(18只)、EPO组(18只);假手术组、SAH组合EPO组根据取材时间有分为1、3、7 d三亚组,每亚组6只。采用枕大池二次注血法建立SAH模型。EPO组建模成功后30 min内腹腔注射EPO(3000 IU/kg,1次/d)。建模成功后1、3、7 d采用取材基底动脉行HE染色测量基底动脉管径及管壁厚度,采用免疫组化检测基底动脉血管壁核转录因子-κB(NF-κB)及内皮细胞型一氧化碳合酶(e NOS)的表达。结果 SAH后1、3、7 d,假手术组基底动脉管径、管壁厚度、NF-κB和e NOS表达水平与正常组无统计学差异(P>0.05);与假手术组相比,SAH组各时间点基底动脉管径均明显减小(P<0.05),管壁厚度均明显增加(P<0.05),NF-κB表达水平均明显增加(P<0.05),e NOS表达水平均明显降低(P<0.05);与SAH组相比,EPO组基底动脉管径、管壁厚度均明显改善(P<0.05),NF-κB表达水平均明显降低(P<0.05),e NOS表达水平均明显增加。结论 EPO能够显著改善大鼠SAH后CVS,机制可能是通过上调e NOS的表达、抑制NF-κB的表达来实现的。Objective To observe the effect of erythropoietin(EPO)on the cerebral vasospasm after subarachnoid hemorrhage(SAH)and its mechanism in rats.Methods Sixty SD rats were randomly divided into normal(n=6),SAH(n=18),sham operation(n=18)and treatment groups(n=18).Each rat received intraperitoneal injection of EPO(3000IU/kg)within 30 minutes after SAH in the treatment group and1 day.Each rat in sham and SAH groups received the intraperitoneal injection of isovolumic physiologic saline once a day.Six rats were sacrificed respectively 1,3 and 7 days after establishment of SAH model in SAH,sham and EPO treatment groups.The rats in sham group were sacrificed at 1 day,3 days and 7 days after successful modeling in order to take basilar arteries.The basilar artery spasm was observed after hematoxylin-eosin staining.The expressions of nuclear factor-kappa B(NF-κB)and endothelial nitric oxide synthase(eNOS)in the walls of the basilar arteries were detected by immunohistochemical technique in all the groups 1,3 and 7 days after SAH.Results The diameters of the basilar arteries were significantly bigger in normal and sham operation groups than that in the EPO treatment group(P<0.05),which was significantly bigger than that in the SAH group 1,3 and 7 days after SAH(P<0.05).The basilar arteries walls were significantly thicker in SAH group than that in the EPO treatment group(P<0.05),which was significantly thicker than those in the normal and sham operation groups 1,3 and 7 days after SAH(P<0.05).The levels of eNOS expressions were significantly higher in the normal and sham operation groups than that in EPO treatment group(P<0.05),which was significantly higher than that in the SAH group 1,3 and 7 days after SAH(P<0.05).The level of NF-κB expressions were significantly lower in the normal and sham operation groups than that in EPO treatment group(P<0.05),which was significantly lower than that in SAH group 1,3 and 7 days after SAH(P<0.05).Conclusions That EPO relives cerebral vasospasm after SAH may be achieved by upregulating

关 键 词：蛛网膜下腔出血 脑血管痉挛 促红细胞生成素 核转录因子-ΚB 内皮细胞型一氧化碳合酶 大鼠 

分 类 号：R743.9[医药卫生—神经病学与精神病学]