大鼠正畸牙移动过程中压力侧牙周组织缺氧诱导因子-1α的表达  

Expression of hypoxia-inducible factor 1α in the pressure-side periodontium during orthodontic tooth movement in rats

作　　者：何正权 杨凯[2] HE Zhengquan;YANG Kai(Department of Orthodontics,Changsha Stomatological Hospital,Changsha 410004,China;Department of Orthodontics,Beijing Stomatological Hospital,Beijing 100050,China)

机构地区：[1]长沙市口腔医院正畸科,湖南长沙410004 [2]首都医科大学附属北京口腔医院正畸科,北京100050

出　　处：《口腔疾病防治》2018年第4期227-230,共4页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：湖南省卫计委科研基金项目(B2016237);北京市教委科技计划面上项目(KM201310025021)

摘　　要：目的观察大鼠正畸牙移动过程中压力侧牙周组织缺氧诱导因子-1α(hypoxia-inducible factor 1α,HIF-1α)的表达。方法将50只6周龄雄性SD大鼠随机分为10组,每组5只。以左上第一磨牙作为实验牙,加力10~15 g,分别在加力0、1、3、6、12 h,1、3、7、14、21 d后制备不同时间点牙周组织切片,进行HE染色和HIF-1α免疫组化染色,利用image-pro plus图像分析系统对染色后的切片做定位定量分析。结果正畸牙移动过程中压力侧牙周组织HIF-1α的表达发生变化。加力1 h后牙周膜HIF-1α表达立即增加,3 h达到小高峰,随后降低,12 h后表达再次增加,1 d后达到最高峰,然后逐渐降低接近加力前的水平(P<0.05)。结论大鼠正畸牙移动过程中,压力侧牙周膜不同时间HIF-1α表达均有差异。Objective To assess hypoxia-inducible factor 1α(HIF-1α)expression levels in the periodontal tissues of the pressure side during orthodontic tooth movement in rats.Methods A total of 50 male 6-week-old Sprague Dawley(SD)rats were randomly divided into 10 groups of 5 rats each.The upper left first molar was the experimental tooth and was pulled mesially with an orthodontic force of 10-15 g for 0,1,3,6,or 12 h,or 1,3,7,14,or 21 d.Routine five-micrometer paraffin-embedded tissue sections were processed for HE staining and immunohistochemical staining for HIF-1α.The Image-Pro Plus system was used to quantitatively analyze the stained slices.Results The expression of HIF-1αin the periodontal tissue of the pressure side changed during the process of orthodontic tooth movement.The expression of HIF-1αincreased immediately after loading for 1 h,reached a small peak at 3 h,and then decreased.After 12 h,the expression increased again,reached a peak after 1 d,and then gradually decreased to near the pre-loading level(P<0.05).Conclusion There were differences in expression of HIF-1αin different groups in the periodontal tissues of the pressure side during orthodontic tooth movement in rats.

关 键 词：正畸 牙齿移动 牙周组织改建 缺氧 诱导因子1α 

分 类 号：R783.5[医药卫生—口腔医学]