光周期和红光对光唇鱼卵巢发育和机体氧化应激状态的影响  

The effect of photoperiod and red light on ovary development and oxidative stress status in a stream fish(Acrossocheilus fasciatus)

作　　者：颉志刚[1,2] 鲁纪刚 XIE Zhigang;LU Jigang(College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321004,China;Key Lab of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province,Jinhua 321004,China)

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]浙江省野生动物生物技术与保护利用重点实验室,浙江金华321004

出　　处：《渔业研究》2018年第2期111-119,共9页Journal of Fisheries Research

基　　金：浙江省公益技术应用研究计划国际合作项目(NO.2017C34006);金华市科学技术研究计划项目(NO.2017-2-020)

摘　　要：为探究光周期和红光照射对光唇鱼(Acrossocheilus fasciatus)性腺发育的影响,本研究利用不同光周期(12L∶12D和16L∶8D)和光色(白色和红色)设计两因素两水平交互实验,比较了雌性光唇鱼的性腺指数(GSI)、各期卵母细胞比例、血浆褪黑素(MT)含量、肌肉和肝脏组织丙二醛(MDA)含量及超氧化物歧化酶(SOD)活力的变化。结果表明:红光(626 nm)长光照组雌性光唇鱼GSI和Ⅲ期卵母细胞比例最大,而白光短光照组最小(P<0.05);红光长光照组夜间血浆MT水平最高,而白光短光照组最低(P<0.05);光周期和光色对肌肉组织MDA含量和组织SOD酶活力均无显著性影响(P>0.05),但长光照和红光均可显著增加肝组织MDA含量(P<0.05);光周期和光色在多个指标中存在显著的交互作用。总之,红光结合长光周期可协同加速雌性光唇鱼的卵巢发育并提高血浆MT水平,这暗示MT在繁殖调控方面可能充当了重要角色。The aim of the study was to explore the effects of photoperiod and red light irradiation on ovarian development of a stream fish,Acrossocheilus fasciatus.The interactive experiment with two factors and two levels was designed basing on the different photoperiods(12L∶12D and 16L∶8D)and light colors(white and red).Gonadosomatic index(GSI),the proportion of developing oocytes in each stage,plasma melatonin(MT)levels,and malondialdehyde(MDA)contents and superoxide dismutase(SOD)activity of muscle and liver were investigated in the pre-breeding females.The results showed that the maximum of GSI and the percentage of developing oocyte at stageⅢoccurred in the fishes under long photoperiod(LP)with red light,but the minimum in those under shot photoperiod(SP)with white light(P<0.05)(Fig.2).The maximum level of night plasma MT was found under LP with red light condition,while the minimum level was under the SP with white light condition(P<0.05)(Fig.3).Neither photoperiod nor light color had a significant influence on muscular MDA contents and SOD activities of muscle and liver.However,hepatic MDA contents were significant increased by LP and red light(P<0.05)(Fig.4).The interaction effect between photoperiods and light colors was detected in the most biological indicators.As a conclusion,long photoperiod with red light could significantly improve ovary development accompanied with the increase of plasma MT level,which suggested that MT played a crucial role in regulating reproduction in this species.

关 键 词：光唇鱼 光周期 红光 性腺发育 褪黑素 氧化应激 

分 类 号：S917.4[农业科学—水产科学]