樟子松2种块菌属外生菌根形态描述和分子鉴定  

Morphology and Identification of Truffles in the Soil of Pinus sylvestris var. mongolica Forest

作　　者：张文泉[1] 罗国涛[1] 闫伟[2] ZHANG Wen-quan;LUO Guo-tao;YAN Wei(Kaili University of Guizhou,Kaili 556011,China;College of Forestry,Inner Mongolia Agricultural University,Hohhot 010019,China)

机构地区：[1]凯里学院,贵州凯里556011 [2]内蒙古农业大学林学院,内蒙古呼和浩特010019

出　　处：《浙江林业科技》2018年第1期6-10,共5页Journal of Zhejiang Forestry Science and Technology

基　　金：贵州省科技厅基础研究项目(黔科合基础[2018]1139号);贵州省科技厅基础研究项目(黔科合基础[2017]1165号);贵州省科技厅联合基金项目(黔科合LH字[2014]7241号);贵州省科技厅联合基金项目(黔科合LH字[2015]7752号);凯里学院博士专项课题(凯院合BS201339号)

摘　　要：利用外部形态和解剖结构特征进行对比,选出樟子松Pinus sylvestris var.mongolica 2个类似块菌属Tuber的外生菌根类型,再采用CTAB法对其进行基因组DNA提取,并利用真菌特有引物ITS1F和ITS4,在PCR基础上对其r DNA的ITS区段进行碱基序列测定,并与Gen Bank数据库中已知的相关序列进行Blast比对得出:类型1,类型2均为块菌属的外生菌根,类型1可鉴定到种,为辽东块菌Tuber liaotongense。In May and October of 2014 and 2015,collections of soil sample were implemented under Pinus sylvestris var.mongolica forests in 6 forest farms in the Nei Monggol Autonomous Region.Roots with mycorrhiza were selected from soil sample and two types of truffle were described and identified.The extraction of genomic DNA of two truffles by CTAB method,and on the basis of PCR,ITS regions of DNA of the macrofungi were amplified and sequenced by using the special pairs of primers ITS1F/ITS4.Their sequences were compared with those registered in GenBank database.They were belonged to Tuber,and one of them was T.liaotongense.

关 键 词：外生菌根 形态特征 分子鉴定 樟子松 块菌 

分 类 号：S791.253[农业科学—林木遗传育种]