海南山竹果实不同部位多酚提取物抗氧化活性、乙酰胆碱酯酶和α-葡萄糖苷酶抑制活性研究  

Antioxidant activity, acetylcholine esterase and α-Glucosidase inhibitory activity of the poly-phenol extractives from peel and pulp of Garcinia mangostana fruit grown in Hainan province

作　　者：谭琳[1] 周兆禧[1] 王甲水[1] 马伏宁[1] 康由发 TAN Lin;ZHOU Zhao-xi;WANG Jia-shui;MA Fu-ning;KANG You-fa(Haikou Experimental Station,Chinese Academy of Tropical Agricultural Sciences/Hainan Key Laboratory of Banana Genetic Improvement,Haikou 570102,China;Management Committee of Chengxi Campus,Hainan University,Haikou 571101,China)

机构地区：[1]中国热带农业科学院海口实验站/海南省香蕉遗传改良重点实验室,海南海口570102 [2]海南大学城西校区管委会,海南海口571101

出　　处：《广东农业科学》2018年第1期102-107,共6页Guangdong Agricultural Sciences

基　　金：中国热带农业科学院海口实验站科研启动项目(HKZKY140204);中国热带农业科学院基本科研业务费(1630092017010)

摘　　要：了解海南山竹果皮和果肉的多酚含量及其抗氧化活性、乙酰胆碱酯酶抑制活性和α-葡萄糖苷酶抑制活性,为引导消费、开发利用奠定基础。以采自海南五指山的山竹果实为材料,用95%的乙醇提取山竹果皮和果肉多酚,采用福林-酚法测定总酚含量。对获得的山竹果皮和果肉多酚提取物进行羟自由基(·OH)、DPPH自由基、ABTS自由基清除率以及乙酰胆碱酯酶抑制活性和α-葡萄糖苷酶抑制活性测定。结果表明,山竹果皮多酚含量很高,达21.36mg/g(干重),对·OH、DPPH自由基和ABTS+·均具有良好的清除活性,且呈现浓度依赖性,IC50分别为37.39、41.34、41.37μg/mL;山竹果皮多酚对乙酰胆碱酯酶和α-葡萄糖苷酶也具有很强的抑制活性,且呈现浓度依赖性,IC50分别为29.46、7μg/mL;山竹果肉多酚含量较低,为0.388mg/g(干重),清除DPPH自由基、ABTS+·和·OH活性较弱,但表现出浓度依赖效应。山竹果肉多酚对α-葡萄糖苷酶具有中等程度的抑制活性(IC50为2.24mg/mL),对乙酰胆碱酯酶抑制活性较弱。山竹果皮多酚具有良好的抗氧化性、乙酰胆碱酯酶抑制活性和α-葡萄糖苷酶抑制活性;山竹果肉多酚具有中等程度的α-葡萄糖苷酶抑制活性,一定的抗氧化性和乙酰胆碱酯酶抑制活性。The total phenolics were extracted from the peel and pulp of Garcinia mangostana fruit with 95%ethanol,and were subjected to quantification using Folin phenol method.The antioxidant activities of the peel and pulp extracts were evaluated by using Hydroxyl,DPPH and ABTS radical method,as well the acetylcholine esterase andα-glucosidase inhibitory activity were assayed.The results suggested that the peel extract contained a very high level of total phenolics(21.36 mg/g DW)with the high capacity to scavenge DPPH,ABTS and hydroxyl radical in a concentration-dependent manner.The IC50 were 37.39,41.34,41.37μg/mL,respectively.It also exhibited the strong inhibitory activity against acetylcholine esterase andα-Glucosidase in a concentration-dependent manner with IC50 of 29.46 and 7μg/mL,respectively.Unlike those from peel,the extracts from pulp contained much lower level of total phenolics(0.388 mg/g DW).The antioxidant activity and inhibitory activity against acetylcholine esterase were also found to be weaker in the extracts from the pulp when compared with those from peel,which displayed modestα-glucosidase inhibitory activity with IC50 of 2.24 mg/mL.It was concluded that the poly-phenols from the peel of Garcinia mangostana fruit had strong antioxidant potential andα-glucosidase inhibitory activity as well as acetylcholine esterase inhibitory activity,whereas,those from the pulp indicated the modest level of activities.

关 键 词：山竹 果实多酚 抗氧化活性 自由基清除活性 乙酰胆碱酯酶抑制活性 α-葡萄糖苷酶抑制活性 

分 类 号：S667.9[农业科学—果树学]