不同拉伸力加载培养对人血管内皮细胞增殖情况的影响和意义  

Effect of Different Tensile Forces on the Proliferation of Cultured Human Vascular Endothelial Cells and Their Significance

作　　者：李巍 陈博 杜广刚 岑瑛[2] Li Wei;Chen Bo;Du Guanggang;Cen Ying(Department of Burn,Sichuan Academy of Medicine Sciences&Sichuan Provincial People's Hospital,Chengdu 610072,China;Department of Plastic and Burn Surgery,West China Hospital of Sichuan University,Chengdu 610041,China)

机构地区：[1]四川省医学科学院.四川省人民医院烧伤科,成都610072 [2]四川大学华西医院整形烧伤科,成都610041

出　　处：《成都医学院学报》2018年第2期182-185,188,共5页Journal of Chengdu Medical College

基　　金：四川省卫计委科研课题(No:17PJ154)

摘　　要：目的探讨外在不同强度机械拉伸力加载对体外培养人血管内皮细胞生长的影响和相关生长因子的表达情况,以及其在临床负压治疗中的指导意义。方法对体外培养的人脐静脉血管内皮细胞加载不同拉伸力干预,实验分6组,分别加载FX-5000T应力系统不同大小拉伸力(拉伸度分别为0、5%、10%、15%、20%、30%)后,利用CCK-8法检测细胞增殖情况,qRT-PCR检测血管形成因子相关基因VEGF、Flk-1、Tie-2表达水平,提取相关蛋白WB检测Flk-1、Tie-2蛋白表达水平。结果各应力加载培养组细胞活性下降,且应力越高细胞活性越低;各应力组细胞内VEGF、Flk-1、Tie-2基因的表达呈不同程度下调趋势,且应力越高,VEGF、Flk-1、Tie-2的表达越低;各应力组细胞内Flk-1、Tie-2的蛋白表达呈下调趋势,且呈现一定的剂量依赖性,即应力越高,Flk-1、Tie-2的蛋白表达越低。结论外在拉伸力对人血管内皮细胞生长增殖有一定影响,且长时间超生理范围的拉伸力对人血管内皮细胞的增殖有明显抑制作用。Objective To study the effect of different external mechanical tensile forces on the growth of the human vascular endothelial cells cultured in vitro and the expression of the related growth factors and provide the guiding significance in the clinical negative pressure treatment.Methods The human umbilical vein endothelial cells cultured in vitro were loaded with different tensile forces and divided into 6 groups with the tensile strength of 0,5%,10%,15%,20%and 30%respectively with the help of the stress system of FX-5000T.The cell proliferation was assayed by CCK-8,the expression of the growth factors including VEGF,Flk-1 and Tie-2 were tested by qRT-PCR,and the related proteins were detected by the WB test.Results The cell viability in each stress-loaded group decreased significantly,and the increase of tensile force resulted in the decrease of the cell activity.The expression levels of VEGF,Flk-1 and Tie-2 in the groups with different tensile forces showed the downward trend to different degrees and they decreased with the increase of the tensile force.The expression levels of Flk-1 and Tie-2 in each group showed a downward trend in a dose-dependent manner and they decreased with the increase of the tensile force.Conclusion The external tensile force has a certain effect on the proliferation of the proliferation of human vascular endothelial cells and the long-time tensile force in the super-physiological range has a significant inhibitory effect on the proliferation of human vascular endothelial cells.

关 键 词：拉伸力血管 内皮细胞 培养 增殖 

分 类 号：R364.3[医药卫生—病理学]