和厚朴酚对咪喹莫特诱导小鼠银屑病的干预作用  

Anti-psoriatic effects of honokiol in imiquimod induced mouse model

作　　者：钟淇滨 祝曙光[2] 陆少君 蔡肇栩 臧林泉 ZHONG Qi-bin;ZHU Shu-guang;LU Shao-jun;CAI Zhao-xu;ZANG Lin-quan(Dept of Pharmacology,School of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China;Dept of Cardiothoracic Surgery,the First Affiliated Hospital of Guangdong Pharmaceutical University,Guangzhou 510006,China)

机构地区：[1]广东药科大学药学院药理系,广东广州510006 [2]广东药科大学附属第一医院胸心外科,广东广州510006

出　　处：《中国药理学通报》2018年第5期626-631,共6页Chinese Pharmacological Bulletin

基　　金：广东省自然科学基金资助项目(No 2017A030313856)

摘　　要：目的探索和厚朴酚达到血药稳态浓度后,对咪喹莫特诱导小鼠银屑病模型的干预作用及其机制。方法将BALB/c小鼠随机分为空白对照组、模型对照组、地塞米松阳性组、脂质体溶剂对照组及和厚朴酚高、中、低剂量组。采用银屑病皮损面积和疾病严重程度(PASI)评分观察疾病进展,HE染色观察皮肤细胞形态学改变,测量皮肤厚度,免疫组织化学法半定量分析IL-17、IL-23、JAK、STAT3、NF-κB、TNF-α的表达。结果模型组小鼠皮肤肥厚,长出鳞屑,表皮出现棘层增生变厚,真皮层出现炎症性浸润和微脓肿,IL-17、IL-23、JAK、STAT3、NF-κB、TNF-α均比正常组表达增加。与模型组相比,和厚朴酚能减轻表皮厚度,减少炎症性浸润和微脓肿,降低IL-17、IL-23、JAK、STAT3、NF-κB、TNF-α的表达,并且有剂量依赖性。结论和厚朴酚能通过抑制IL-17/IL-23炎症轴,改善咪喹莫特诱导的小鼠银屑病样皮损变化。Aim To explore the anti-psoriatic effects of honokiol on the mouse model induced by imiquimod and the underlying mechanism.Methods The mice were randomly divided into control group,model group,liposome solvent control group,dexamethasone positive group and honokiol high,medium,low dose groups.The progress of the disease was observed by the psoriasis area and severity index(PASI).The morphological changes of the skin cells were observed by HE staining,and epidermis thickness was measured.The expression of IL-17,IL-23,JAK,STAT3,TNF-αand NF-κB was semi-quantitively analyzed by immunohistochemistry.Results Compared to model group,the scaling and thickness of honokiol treated groups were alleviated.Inflammatory infiltration and micro abscess were reduced.The expressions of IL-17,IL-23,JAK,STAT3,TNF-αand NF-κB in model group were higher than those in honokiol-high and honokiol-medium group in a dose-dependent manner.Conclusion Honokiol can inhibit imiquimod-induced psoriasis-like lesions in mice by inhibiting the IL-17/IL-23 inflammatory axis.

关 键 词：和厚朴酚 咪喹莫特 银屑病 IL-17 IL-23 JAK STAT3 NF-κB TNF-α 

分 类 号：R-332[医药卫生] R284.1