钾通道阻滞剂四乙基铵敏感性钾电流在肺高压大鼠肺动脉平滑肌细胞中的变化  

Alteration of tetraethylammonium-sensitive potassium current in pulmonary artery smooth muscle cells of pulmonary hypertension rats

作　　者：张双双 穆云萍[1] 贺志成 颜晓晓 林默君[1] ZHANG Shuang-shuang;MU Yun-ping;HE Zhi-cheng;YAN Xiao-xiao;LIN Mo-jun(Dept of Physiology and Pathophysiology,the Key Lab of Fujian Province Universities on Ion Channel and Signal Transduction in Cardiovascular Diseases,Fujian Medical University,Fuzhou 350108,China)

机构地区：[1]福建医科大学基础医学院生理学与病理生理学系,心血管病离子通道信号调控福建省高校重点实验室,福建福州350108

出　　处：《中国药理学通报》2018年第5期664-669,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No31571179,31371165);福建省自然科学基金资助项目(No2015J01313)

摘　　要：目的研究大鼠肺动脉平滑肌细胞(pulmonary arterial smooth muscle cells,PASMCs)中电压依赖性钾通道(voltagedepending potassium channel,K_V)电流在肺高压(pulmonary hypertension,PH)中的变化,及钾通道阻滞剂四乙基铵(tetraethylammonium,TEA)对K_V电流的影响。方法采用全细胞膜片钳技术记录PASMCs的K_V电流,观察PH大鼠中K_V电流的变化,以及TEA对K_V的影响。结果野百合碱(monocrotaline,MCT)、慢性低氧(chronic hypoxia,CH)明显减小了大鼠PASMCs上记录到的K_V电流密度;TEA干预能够明显降低正常和PH大鼠PASMCs的K_V电流,并且TEA对K_V电流的抑制作用在PH大鼠中明显减小。结论在CH、MCT诱导的两种PH大鼠模型中,PASMCs的K_V开放程度被明显抑制,而且其对应的TEA敏感性K_V电流明显减小。Aim To investigate the alteration of voltage-depending potassium channel(K V)current in pulmonary arterial smooth muscle cells(PASMCs)of pulmonary hypertension(PH)rats,and the effect of tetraethylammonium(TEA,a blocker of K V)on potassium channel current in different PH models.Methods The whole-cell patch clamp techniques were applied to record the K V currents from PASMCs cultured with Ham’s F-12(1%FBS).Furthermore,the effects of TEA on the K V currents were examined in different PH models.Results The whole-cell K V currents were obviously inhibited in PASMCs of chronic hypoxia(CH)and monocrotaline(MCT)-treated rats.TEA significantly decreased the whole-cell K V currents in PASMCs of control and PH rats,and the inhibitory effect of TEA was dramatically reduced in PH group.Conclusions The degree of the voltage-dependent potassium channels opening is significantly inhibited in PASMCs of CH and MCT-treated rats,accordingly,the TEA-sensitive K V currents obviously decrease.

关 键 词：四乙基铵 电压依赖性钾通道 肺高压 肺动脉平滑肌细胞 野百合碱 慢性低氧 

分 类 号：R-332[医药卫生] R322.121