人U87-MG脑胶质瘤细胞裸鼠原位移植模型的建立  

Establishment of orthotopic implantation model of human U87-MG brain glioma cell line in nude mice

作　　者：王艳华[1] 楚建杰[1] 李子敏 胡娜平 李会会[1] 郑建民 张彩勤 师长宏 杨志福[1] 奚苗苗[1] 文爱东[1] 翁琰[1] WANG Yan-hua;CHU Jian-jie;LI Zi-min;HU Na-ping;LI Hui-hui;ZHENG Jian-min;ZHANG Cai-qin;SHI Chang-hong;YANG Zhi-fu;XI Miao-miao;WEN Ai-dong;WENG Yan(Dept of Pharmacy,,Xijing Hospital,Xi’an 710032,China;Dept of Radiology,Xijing Hospital,Xi’an 710032,China;Experimental Animal Center,Air Force Military Medical University,Xi’an 710032,China)

机构地区：[1]西京医院药剂科,陕西西安710032 [2]西京医院放射科,陕西西安710032 [3]空军军医大学实验动物中心,陕西西安710032

出　　处：《中国药理学通报》2018年第5期735-739,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No81303264)

摘　　要：目的应用不同数量U87-MG细胞接种裸鼠脑内,建立裸鼠脑胶质瘤模型,观察其生长特性。方法采用立体定向技术,分别将3.0×10^(10)·L^(-1)、4.0×10^(10)·L^(-1)、5.0×10^(10)·L^(-1)浓度的人脑胶质瘤细胞U87-MG单细胞悬液,接种于18只♂裸鼠的右侧尾状核区,每只接种体积为5μL,另取6只裸鼠作为对照,接种同体积Hanks液。观察不同接种量实验鼠的生存状态、成瘤情况及脏器转移灶、带瘤生存期,测量肿瘤的最大径,计算肿瘤体积,将获取的全脑标本制作病理切片,行HE染色和免疫组化检查。结果各接种量的实验组成瘤率均为100%,未见颅外转移病灶,3组裸鼠的平均生存时间分别为(46.50±3.27)d、(38.50±3.28)d、(30.67±3.51)d;病理学检查符合人脑胶质瘤细胞的形态学特征和免疫表型;免疫组化GFAP和S-100蛋白呈阳性表达。结论立体定向脑内定量注射U87-MG细胞制备的人脑胶质瘤裸鼠原位移植模型成瘤率高、颅内生长稳定、颅外远隔部位转移率低,与人脑胶质瘤病理学及形态学特征相似,能为研究胶质瘤的发生、发病机制、生物学特性以及探寻有效的治疗措施提供可靠的动物模型。Aim To establish human U87-MG glioma model in nude mice brain and to observe the characteristics of the tumor growth.Methods Human U87-MG glioma cells were cultured in vitro.5μL of cell suspension containing 3.0×10 10·L-1,4.0×10 10·L-1 and 5.0×10 10·L-1 respectively was inoculated into the right caudate nucleus of 18 male nude mice brain under the guidance of stereotaxic apparatus,separately,whereas another 6 nude mice as the control group,were inoculated into the same volume of Hanks solution.The moving and survival state of rats with gliomas were observed.The examinations of the tumors formation,volumes,metastasis and histopathology were performed and the obtained brain samples were stained with HE and immunohistochemistry.Results All the tested rats of different inoculation doses developed brain tumors without extracranial metastasis.The mean survival time of three groups was(46.50±3.27)d,(38.50±3.28)d and(30.67±3.51)d,respectively.The tumors showed the similar morphological features and immunophenotype to human glioma.There was positive expression of GFAP and S-100 in the tumors.Conclusions The orthotopic implantation model of human U87-MG glioma,by inoculating quantitative U87-MG cells stereotaxically into the brains of the nude mice,is successfully established with 100 yield of intracranial tumor and no extracranial growth extension.It resembles the histopathological and morphological features of human glioma,which can be used as a reliable animal model for the study of the tumorigenesis,pathogenesis,biological characteristics and therapy of glioma.

关 键 词：脑胶质瘤 原位移植 裸鼠 动物模型 U87-MG细胞 HE染色 免疫组化 

分 类 号：R-332[医药卫生] R363-332