埃兹蛋白通过L1细胞黏附分子信号通路促进肺癌转移  

Ezrin promotes metastasis of lung cancer via L1CAM signaling pathway

作　　者：冯辉[1] 金明根[1] 金铁峰[2] FENG Hui;JIN Minggen;JIN Tiefeng(Department of Intensive Care Medicine,Affiliated Hospital of Yanbian University, Yanji,Jilin 133000,China;Tumor Research Center,Yanbian University,Yanji,Jilin 133002,China)

机构地区：[1]延边大学附属医院重症医学科,吉林延吉133000 [2]延边大学肿瘤研究中心,吉林延吉133002

出　　处：《重庆医学》2018年第12期1589-1591,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(81660394)

摘　　要：目的研究埃兹蛋白(Ezrin)促进人肺癌发生转移的分子机制。方法通过RT-PCR、Western blot检测肺癌细胞系中Ezrin的表达;划痕试验检测Ezrin对肺癌细胞系迁移能力的影响;Western blot检测Ezrin调控L1细胞黏附分子(L1CAM)的机制。结果 Western blot结果显示,与低转移肺癌细胞系H460、EBC-1比较,高转移细胞系95D及PC9具有更高的Ezrin蛋白表达(P<0.05);用基因方法沉默95D细胞Ezrin表达后(siEzrin-95D),细胞划痕试验结果发现与95D细胞比较,siEzrin-95D细胞的迁移能力明显减弱(P<0.05)。与95D及H460细胞比较,基因沉默95D及H460细胞Ezrin表达后,其L1CAM的表达明显下调(P<0.05)。结论 Ezrin可能通过调节L1CAM的表达促进肺癌转移。Objective To investigate the molecular mechanism of Ezrin in promoting human lung cancer metastasis.Methods The expression of Ezrin in lung cancer cell lines was detected by using RT-PCR and western blot;the cell scratch test was used to detect the effect of Ezrin on the migration ability of lung cancer cells;the mechanism of Ezrin for regulating L1CAM was detected by western blot.Results The western blot detection results showed that compared with the low metastatic lung cancer cell line H460 and EBC-1,the expression of Ezrin protein the high metastatic lung cancer cell line 95D and PC9 were higher(P<0.05).After silencing the expression of Ezrin in 95D cells(siEzrin-95D)by using the genetic method,the cell scratch test showed that the migration ability of siEzrin-95D cells was significantly weakened compared with 95D cells(P<0.05).Compared with 95D cells and H460 cells,after genetically silencing the Ezzin expression in 95D and H460 cells,the L1CAM expression was significantly down-regulated(P<0.05).Conclusion Ezrin promotes lung cancer metastasis possibly by regulating the expression of L1CAM.

关 键 词：肺肿瘤 埃兹蛋白 L1细胞黏附分子 转移 

分 类 号：R361.1[医药卫生—病理学]