MBP-1在人食管癌细胞株Ec109细胞增殖、凋亡和侵袭中的作用  

Role of MBP-1 in proliferation,apoptosis and invasion of human esophageal cancer cells Ec109

作　　者：陈家骅[1] 李元明[1] 王磊[1] 叶力波 伊斯拉木江 木沙由夫[1] 哈里木[1] 齐海[1] CHEN Jiahua;LI Yuanming;WANG Lei;YE Libo;YI Silamujiang;MU Shayoufu;HA Limu;QI Hai(Department of Cardiothoracic Surgery,Second Affiliated Hospital of Xinjiang Medical University,Urumchi,the Xingjiang Uygur Autonomous Region 830063,China)

机构地区：[1]新疆医科大学第二附属医院心胸外科,乌鲁木齐830063

出　　处：《重庆医学》2018年第10期1318-1321,共4页Chongqing medicine

摘　　要：目的探讨癌症-MYC基因(C-myc)启动子结合蛋白1(MBP-1)在人食管癌细胞株Ec109细胞增殖、凋亡和侵袭中的作用。方法培养人食管癌细胞系Ec109,分为MBP-1模拟物组、siRNA-MBP-1组、阴性对照组和空白对照组,采用四甲基偶氮唑蓝(MTT)检测各组细胞增殖,流式细胞仪检测细胞凋亡及细胞周期,Transwell检测细胞侵袭能力,Western blot检测与细胞周期相关的C-myc、周期蛋白D1(Cyclin D1)和周期蛋白E(Cyclin E)。结果与阴性对照组和空白对照组相比,转染MBP-1模拟物可使食管癌细胞Ec109中MBP-1表达上调(P<0.05),食管癌细胞增殖能力降低,凋亡比例升高,G0/G1期细胞比例降低,侵袭细胞数减少,细胞中C-myc、Cyclin D1和Cyclin E蛋白表达被抑制;沉默MBP-1后,siRNA-MBP-1组食管癌细胞Ec109中MBP-1表达下调,食管癌细胞增殖能力增加,凋亡比例下降,G0/G1期细胞比例升高,侵袭细胞数增加,细胞中C-myc、Cyclin D1和Cyclin E蛋白表达上调。结论 MBP-1与人食管癌细胞株Ec109细胞增殖、凋亡、侵袭能力密切相关,其机制可能与细胞周期异常有关。Objective To investigate the role of MBP-1 in proliferation,apoptosis and invasion of human esophageal cancer cells Ec109.Methods The human esophageal cancer cells Ec109 were cultured,and divided into the MBP-1 mimics group,siRNA-MBP-1 group,negative control group and blank control group.The cell proliferation activity of each group was detected by tetrazolium blue(MTT)method;flow cytometry was used to detect cell apoptosis and cell cycle;Transwell assay was used to detected the invasion ability and the expressions of cellular cycle related C-myc,Cyclin D1 and Cyclin E were detected by western blot.Results Compared with the negative control group and blank control group,the expression of MBP-1 in esophageal cancer cells Ec109 of the MBP-1 mimics group was up-regulated(P<0.05),the proliferation ability of esophageal cancer cells was decreased,increased the proportion of apoptosis,decreased the proportion of G 0/G 1 phase cells,inhibited the number of invasive cells was decreased and the expressions of C-myc,Cyclin D1 and Cyclin E proteins.After silencing MBP-1,the expression of MBP-1 in esophageal cancer cells Ec109 in the siRNA-MBP-1 group was down-regulated,the proliferation ability of esophageal cancer cells was increased,the proportion of apoptosis was decreased,the proportion of G 0/G 1 phase cells was increased.the number of invasive cells was increased and the expressions of C-myc,Cyclin D1 and Cyclin E proteins were up-regulated.Conclusion MBP-1 is closely correlated with the cell proliferation,cell apoptosis and invasion ability of human esophageal cancer cell line Ec109,and its mechanism might be related to cell cycle abnormality.

关 键 词：人食管癌Ec109细胞 MBP-1 生物学功能 细胞周期 

分 类 号：R735.1[医药卫生—肿瘤]