生鲜乳中菌落总数检测方法的比较研究  

Comparative Study on Colony Count Detecting Methods in Raw Milk

作　　者：张三粉[1] 王丽芳[1] 杜琳 宋洁[1] 冯小慧 康博洋 崔艳[1] 骆洪[1] ZHANG San-fen;WANG Li-fang;DU Lin;SONG Jie;FENG Xiao-hui;KANG Bo-yang;CUI Yan;LUO Hong(Laboratory of Quality and Safety Risk Assessment for Agricultural Products(Hohhot),Ministry of Agriculture,Institute of Resources Environment and Detection Technology,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)

机构地区：[1]内蒙古农牧业科学院资源环境与检测技术研究所农业部农产品质量安全风险评估实验室(呼和浩特),内蒙古呼和浩特010031

出　　处：《畜牧与饲料科学》2018年第4期18-20,共3页Animal Husbandry and Feed Science

基　　金：国家农产品质量安全风险评估重大专项(GJFP201800803);内蒙古农牧业科学院创新基金项目(2017CXJJM09)

摘　　要：分别利用Peelplate 3M测试片法和国标法对采集自内蒙古呼伦贝尔市、通辽市、呼和浩特市的生鲜乳样品中的菌落总数进行测定,采用方差分析法对2种方法的测定结果进行比较;利用Peelplate 3M测试片法,分别将样品培养24 h和48 h,采用方差分析法对不同培养时间的检测结果进行比较。结果表明,Peelplate 3M测试片法对3个地区生鲜乳样品菌落总数的测定结果与国标法相比均无显著差异(P>0.05);采用Peelplate 3M测试片法测定生鲜乳样品菌落总数时,样品培养24 h的测定结果与培养48 h的测定结果无显著差异(P>0.05)。提示在进行生鲜乳样品菌落总数测定时,Peelplate 3M测试片法可以替代国标法,且采用测试片法测定时,样品培养24 h即可完成菌落总数测定。The Peelplate 3M plate detecting method and national standard method were simultaneously used to determine the colony count in raw milk samples collected from Hulunbuir,Tongliao and Hohhot,respectively,and the detecting results of these two methods were statistically compared with variance analysis.The results of Peelplate 3M plate detecting method with different sample incubation time(24 h and 48 h)were also statistically compared with variance analysis.There were no significant differences in detecting results of colony count in raw milk samples from the three regions between Peelplate 3M plate detecting method and national standard method(P>0.05);when using the Peelplate 3M plate detecting method,there was no significant difference in colony count determination results between the sample incubation duration of 24 h and 48 h(P>0.05).Our results demonstrate that the Peelplate 3M plate detecting method can serve as an alternative to the national standard method in raw milk colony count determination.In addition,the 24 h of sample incubation can produce reliable results in Peelplate 3M plate detecting method.

关 键 词：生鲜乳 菌落总数 Peelplate3M测试片法 国标法 检测结果 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]