电针对慢性疲劳综合征大鼠脑组织转化生长因子-β_1基因转录和蛋白表达的影响  

Effects of Electric Acupuncture on the Transforming Growth Factor-β_1 Protein and Gene Expression in Rats with Chronic Fatigue Syndrome

作　　者：王德龙[1] 杨燕 姜凡[1] 郭静[1] 石天宇 屈媛媛 聂宏[2] 杨添淞[2] WANG De-long;YANG Yan;JIANG Fan;GUO Jing;SHI Tian-yu;QU Yuan-yuan;NIE Hong;YANG Tian-song(Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China;Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学,哈尔滨150040 [2]黑龙江中医药大学附属第一医院神经内科,哈尔滨150040

出　　处：《神经损伤与功能重建》2018年第4期169-171,203,共4页Neural Injury and Functional Reconstruction

基　　金：国家自然科学基金(No.81704170;No.81473761);教育部博士点基金项目(No.20122327110007);黑龙江省中医管理局课题(No.ZHY16-003)

摘　　要：目的:探讨电针治疗对慢性疲劳综合征(CFS)模型大鼠脑组织中TGF-β_1基因转录及蛋白表达的影响。方法:雄性SD大鼠48只,随机分为正常组、模型组、束缚组和针刺组,各12只。模型组采用慢性复合应激因素制备CFS模型;束缚组仅给予与针刺相同时间的捆绑;针刺组给予百会、额区、顶区电针治疗;共15 d。RT-PCR技术检测各组TGF-β_1m RNA的转录水平,Western blot技术检测各组TGF-β_1蛋白表达水平。结果:模型组和束缚组大鼠海马和下丘脑中TGF-β_1m RNA转录水平显著高于正常组(P<0.01),针刺组与正常组差异无统计学意义(P>0.05);针刺组海马和下丘脑中TGF-β_1m RNA转录水平显著低于模型组(P<0.01),束缚组和模型组差异无统计学意义(P>0.05)。正常组中TGF-β_1蛋白表达显著低于其他3组(P<0.01);针刺组大鼠海马和下丘脑中TGF-β_1蛋白表达水平明显低于模型组(P<0.01),束缚组和模型组差异无统计学意义(P>0.05)。结论:电针能够降低CFS模型大鼠海马与下丘脑中TGF-β_1m RNA转录水平和TGF-β_1蛋白表达水平。Objective:To study the effect of electric acupuncture on the transforming growth factor(TGF)-β1 signaling pathways of the chronic fatigue syndrome(CFS)rat model.Methods:Forty-eight SD rats were randomly assigned to the normal group,model group,bound group,and acupuncture group with 12 rats per group.The CFS rat model was established by applying multiple chronic stress factors.The bound group rats were subject to binding for an equal duration of time as that of the acupuncture treatment applied to the acupuncture group rats.Acupuncture group rats were treated for 15 days by electric acupuncture to the Baihui acupoint,frontal lobe area,and parietal lobe area.TGF-β1 mRNA transcription was tested by RT-PCR,and TGF-β1 expression was assessed by Western blot.Results:Model group and bound group rats showed significantly increased TGF-β1 mRNA transcription levels in the hippocampus and hypothalamus compared to normal group rats(P<0.01);there was no significant difference between the acupuncture group and normal group(P>0.05).The acupuncture group showed significantly decreased TGF-β1 mRNA transcription levels compared to the model group(P<0.01)while the bound group and model group showed no significant difference(P>0.05).The normal group displayed lower TGF-β1 expression compared to the other 3 groups(P<0.01).Acupuncture group rats showed significantly decreased TGF-β1 expression levels in the hippocampus and hypothalamus compared to model group rats(P<0.01);the bound group and model group showed no significant difference(P>0.05).Conclusion:Electric acupuncture can reduce TGF-β1 mRNA transcription levels and TGF-β1 protein expression in the hippocampus and hypothalamus of CFS model rats.

关 键 词：慢性疲劳综合征 电针 转化生长因子-Β1 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学]