鸡传染性支气管炎病毒ck/CH/LHB/121042毒株的分离鉴定及分子特征研究  

Isolation, identification and molecular characteristics of avian infectious bronchitis virus ck/CH/LHB/121042 isolate

作　　者：马得莹[1] 姜磊 许丽文[1,2] 刘亮亮 韩宗玺[2] MA Deying;JIANG Lei;XU Liwen;LIU Liangliang;HAN Zongxi(School of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China;Division of Avian Infectious Disease,National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China)

机构地区：[1]东北农业大学动物科学技术学院,哈尔滨150030 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室禽传染病研究室,哈尔滨150001

出　　处：《东北农业大学学报》2018年第2期55-63,共9页Journal of Northeast Agricultural University

基　　金：国家科技支撑计划项目(2015BAD12B03);哈尔滨市科技创新人才研究专项资金项目(2013RFXXJ019);黑龙江省应用技术研究与开发计划项目(PC13S02);教育部博士点基金项目(20122325110016)

摘　　要：鸡传染性支气管炎病毒(infectious bronchitis virus,IBV),严重威胁养禽业健康发展。近年来,我国不断出现新型IBV毒株及变异株,给传染性支气管炎(infectious bronchitis,IB)防控造成困难。监测研究报告显示,IBV感染与多种类型毒株有关,主要以LX4型为主。我国部分地区近期出现LX4型与TW型重组毒株,但鲜有TWⅡ毒株报道,其分子特征和抗原特性目前尚不清楚。通过分离河北省某鸡场疑似IB病鸡肾脏,获得传染性支气管炎病毒ck/CH/LHB/121042,基因组全长27 675 bp,共10个开放阅读框,基因组特征为5'UTR-1a-1b-S-3a-3b-3c-M-5a-5b-N-3'UTR,Spike protein裂解酶识别位点为"Arg-Arg-Phe-Arg-Arg"。病毒基因型鉴定为TWⅡ型,基因组遗传进化分析表明,该毒株与2010年河北分离株ck/CH/LHB/100801亲缘关系最近,推测具有相同进化来源。研究结果对于阐明病毒起源、预测未来产生新型毒株和重组毒株等具有重要意义。Infectious bronchitis virus(IBV),caused considerable damage to poultry industry,exists in a wide range of the world.In recent years,new genotypes of IBV and associated variants appeared frequently in different parts of China,resulting in a major obstacle for the prevention and control of this pathogen.Surveillance studies have reported that widespreadly IBV infection in China is related to multiple types.Of these,LX4(QX-like)is the predominant type circulating in chicken flock.However,in a recent study,recombinant IBV originated from QX-type and TW-type strains was reported in some parts of China.Up to now,a few reports on TWⅡstrains is officially demonstrated in China.As the result,the molecular and antigenic characteristics of TWⅡstrains were not clear.In this study,an avian infectious bronchitis virus,designated as ck/CH/LHB/121042,was isolated and identified from kidney of chickens,which was suspected to be infected with IBV in Hebei Province,China.The result showed that ck/CH/LHB/121042 strain was 27 675 bp,with 10 ORFs,characterized by the 5'-cas-Replicase-S-3a-3b-3c-M-5a-5b-N-poly(A)-3'gene order,and the typical avian coronavirus cleavage site of spike protein contained five consecutive basic amino acids,namely,Arg-Arg-Phe-Arg-Arg.Based upon data arising from S1 sequence and phylogenetic analysis,the isolate was TWⅡgenotype and closely related to ck/CH/LHB/100801 strain.Based on this finding,it was speculated that this IBV might derive from ck/CH/LHB/100801 strain.Retrospective studies,involving molecular characterization,are necessary to clarify the origin of the viruses and are significant for the prediction of new variants and recombinants.

关 键 词：鸡传染性支气管炎病毒 分离 全基因组序列 

分 类 号：S511[农业科学—作物学]