miR-214对PTEN蛋白的影响及其在胃癌细胞中的生物学行为  

Effect of miR-214 on PTEN Protein and Its Biological Behavior in Gastric Cancer Cells

作　　者：周晓刚[1] 王凯[1] 沈小刚[1] 王林[1] 覃先鹏 ZHOU Xiao-gang;WANG Kai;SHEN Xiao-gang;WANG Lin;TAN Xian-peng(Department of Gastrointestinal Surgery,Sichuan Provincial People's Hospital,Sichuan Academy of Medical Sciences,Chengdu 610072,China)

机构地区：[1]四川省医学科学院四川省人民医院胃肠外科,成都610072

出　　处：《解放军医药杂志》2018年第4期20-24,共5页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：国家自然基金青年项目(81301910)

摘　　要：目的探讨miR-214通过上调PTEN蛋白影响胃癌细胞的生物学行为的方式及其分子机制。方法q PCR检测不同胃癌细胞株中miR-214的表达水平和胃癌组织中miR-214和PTEN的表达水平;双荧光素酶实验检测miR-214和PTEN蛋白间的相互关系;MTT增殖实验检测miR-214及PTEN对胃癌细胞增殖能力的影响;Transwell实验检测miR-214及PTEN对胃癌细胞侵袭能力的影响;蛋白免疫印迹检测miR-214对PTEN蛋白表达情况的影响。结果胃癌组织中miR-214表达水平高于癌旁组织,PTEN表达水平低于癌旁组织(P<0.05)。MGC-803细胞株中miR-214表达水平高于MGC-823、SGC-7901(P<0.05)。抑制miR-214的表达后,PTEN蛋白活性相应上调(P<0.05)。miR-214-siRNA组MGC-802细胞株的细胞增殖能力较对照组弱,穿膜细胞数少于对照组(P<0.05)。miR-214-siRNA+PTEN-mimic组MGC-802细胞株细胞增殖率低于miR-214-siRNA组,穿膜细胞数少于miR-214-siRNA组(P<0.05)。结论 miR-214和PTEN蛋白共同作用参与胃癌细胞的生物学行为的调控,miR-214通过上调PTEN蛋白影响胃癌细胞的增殖和侵袭行为。Objective To investigate manner and its molecular mechanism of miR-214 affecting biological behavior in gastric cancer cells by up-regulating PTEN protein.Methods Expressions of miR-214 in different gastric cancer cell lines and miR-214 and PTEN expressions in gastric cancer tissues were detected by quantitative polymerase chain reaction(qPCR).Relationship between miR-214 and PTEN protein was detected by double luciferase assay.MTT proliferation assay was used to detect effects of miR-214 and PTEN on proliferation ability of gastric cancer cells.Transwell test was used to detect effects of miR-214 and PTEN on invasion ability of gastric cancer cells.Western blotting was used to detect effect of miR-214 on PTEN protein expression.Results In gastric cancer tissues,miR-214 expression was significantly higher,while PTEN expression was significantly lower than those in paracancerous tissues(P<0.05).The miR-214 expression in MGC-803 cell line was significantly higher than those in MGC-823 and SGC-7901 cell lines(P<0.05).PTEN protein activity was up-regulated accordingly after inhibiting miR-214 expression(P<0.05).Compared with those in control group,proliferation ability of MGC-802 cell line was weaker,and number of transmembrane cells was less than those in miR-214-siRNA group(P<0.05).Proliferation rate of MGC-802 cell line in miR-214-siRNA+PTEN-mimic group was significantly lower,while number of transmembrane cells was less than those in miR-214-siRNA group(P<0.05).Conclusion Combined action of miR-214 and PTEN protein involve in regulating biological behavior in gastric cancer cells,and miR-214 may affect proliferation and invasion of gastric cancer cells by up-regulating PTEN protein.

关 键 词：胃肿瘤 miR-214 PTEN 细胞增殖 肿瘤浸润 

分 类 号：R735.2[医药卫生—肿瘤]