地西他滨对体外培养人子宫肌瘤细胞增殖及凋亡相关基因表达的影响  

Effects of decitabine on proliferation and apoptosis related-genes expressions of human hysteromyoma cells in vitro

作　　者：刘晓慧[1] 杨晨[1] 许香香[1] 李丹[2] 许慧[1] LIU Xiao-hui;YANG Chen;XU Xiang-xiang;LI Dan;XU Hui(Department of Gynecology,Suzhou Municipal Hospital,Suzhou 215006,China;Department of Gynecology and Obstetrics,the First Affiliated Hospital of Soochow University,Suzhou 215006,China)

机构地区：[1]江苏省苏州市立医院妇科,苏州市215006 [2]苏州大学附属第一医院妇产科,江苏省苏州市215006

出　　处：《广西医学》2018年第6期665-669,共5页Guangxi Medical Journal

基　　金：江苏省苏州市科技发展计划(SYS201255)

摘　　要：目的探讨地西他滨对体外培养人子宫肌瘤细胞增殖及凋亡相关基因表达的影响。方法选取29例子宫肌瘤患者的子宫肌瘤及肌瘤旁正常组织细胞进行原代培养。将子宫肌瘤细胞分为对照组及0.1μmol/L、0.5μmol/L、1.0μmol/L地西他滨组,地西他滨组给予相应浓度的地西他滨进行干预,24 h、48 h、72 h后检测各组细胞增殖及凋亡情况。提取子宫肌瘤细胞、肌瘤旁正常组织细胞及经0.1μmol/L、0.5μmol/L、1.0μmol/L地西他滨干预的子宫肌瘤细胞的总RNA,检测X连锁凋亡抑制蛋白(XIAP)、XIAP相关因子1(XAF1)、P21、P16、细胞周期蛋白D1(Cyclin-D1)m RNA表达水平。结果 0.1μmol/L、0.5μmol/L、1.0μmol/L地西他滨组子宫肌瘤细胞增殖抑制率及细胞凋亡比例依次升高(均P<0.05),3组的抑制率及细胞凋亡比例均随时间延长而升高(均P<0.05)。与瘤旁子宫肌细胞比较,子宫肌瘤细胞的XIAP和Cyclin D1 m RNA表达水平升高,而XAF1、P16和P21 m RNA表达水平减低(均P<0.05);与未经地西他滨处理的子宫肌瘤细胞相比,0.5μmol/L、1.0μmol/L地西他滨组XIAP和Cyclin D1 m RNA表达水平下调而XAF1及P21 m RNA表达水平上调,1.0μmol/L地西他滨组P16 m RNA表达水平上调(均P<0.05)。结论地西他滨可抑制子宫肌瘤细胞的增殖并促进其凋亡,并可抑制XIAP、Cyclin-D1等抗凋亡基因表达并上调XAF1、P16和P21等促凋亡基因表达。Objective To investigate the effects of decitabine on the proliferation and apoptosis related-genes expressions of human hysteromyoma cells in vitro.Methods The cells of hysteromyoma and paratumor tissues from 29 patients with hysteromyoma were collected and were primarily cultured.The hysteromyoma cells were divided into control group,0.1μmol/L decitabine group,0.5μmol/L decitabine group and 1.0μmol/L decitabine group.All decitabine groups were treated with corresponding concentrations of decitabine.After 24,48 and 72 hours,the cell proliferation and apoptosis were detected in each group.The total RNA was extracted from the hysteromyoma cells,paratumor tissue cells,and hysteromyoma cells treated with 0.1,0.5 and 1.0μmol/L decitabine,then the expression levels of X-linked inhibitor of apoptosis protein(XIAP),XIAP-associated factor 1(XAF1),P21,P16 and Cyclin-D1 mRNAs were detected.Results The inhibition rate of cell proliferation and the apoptosis ratio decreased in the order of 0.1μmol/L decitabine group,0.5μmol/L decitabine group and 1.0μmol/L decitabine group(all P<0.05),and increased with time in the three groups(all P<0.05).In the hysteromyoma cells,the expression levels of XIAP and Cyclin D1 mRNAs increased but the expression levels of XAF1,P16 and P21 mRNAs decreased compared to the paratumor tissue cells(all P<0.05).Compared to the hysteromyoma cells without intervention of decitabine,the expression levels of XIAP and Cyclin D1 mRNAs deceased but the expression levels of XAF1 and P21 mRNAs increased in the 0.5μmol/L and 1.0μmol/L decitabine groups,and the expression level of P16 mRNA also increased in the 1.0μmol/L decitabine group(all P<0.05).Conclusion Decitabine can inhibit proliferation and promote apoptosis in hysteromyoma cells,can inhibit the expressions of anti-apoptosis genes(including XIAP and Cyclin-D1)but up-regulate the expressions of apoptosis genes(including XAF1,P16 and P21).

关 键 词：子宫肌瘤 地西他滨 细胞增殖 细胞凋亡 抗凋亡基因 促凋亡基因 

分 类 号：R737.33[医药卫生—肿瘤]