HPV E6/E7 mRNA检测在宫颈病变中应用价值的探讨  

Application value of HPV E6/E7 mRNA testing for cervical lesions

作　　者：贺仁娟 赵敏[2] HE Ren-juan;ZHAO Min(Shanxi Medical University,Shanxi Taiyuan 030001;Maternal and childhealth care hospital of Shanxi province Taiyuan,Shanxi Taiyuan 030013,China)

机构地区：[1]山西医科大学,山西太原030001 [2]山西省妇幼保健院,山西太原030013

出　　处：《实用妇科内分泌电子杂志》2018年第1期43-44,46,共3页Electronic Journal of Practical Gynecological Endocrinology

摘　　要：目的探讨在宫颈病变中HPV E6/E7 m RNA检测的应用价值。方法对104例高危型HPV感染患者取宫颈脱落细胞行HPV E6/E7 m RNA检测以及阴道镜下宫颈活组织活检,以病理诊断结果为金标准分组,进行统计分析。随机抽取30例患者,每位患者均采用两种标本(宫颈脱落细胞和宫颈石蜡包埋组织标本)进行HPV E6/E7 m RNA检测。结果随着宫颈病变程度的加重,HPV E6/E7 m RNA阳性率增加;宫颈脱落细胞标本和宫颈石蜡包埋组织标本都可以用来检测HPV E6/E7 m RNA,两种标本的HPV E6/E7 m RNA阳性率比较,差异无统计学意义(P>0.05)。结论 HPV E6/E7 m RNA阳性率与宫颈病变的严重程度有关。在宫颈癌的筛查中,宫颈脱落细胞和宫颈石蜡包埋组织均可作为HPV E6/E7 m RNA检测的标本。Objective To investigate the application value of HPV E6/E7 mRNA testing for cervical lesions.Methods 104 women with HR-HPV DNA-positive was enrolled and underwent HPV E6/E7 mRNA,colpocopy and biopsy of suspected cervical lesions.Cervical exfoliative cells were analyzed for HPV E6/E7 mRNA.The pathological diagnosis was as the gold standard to group and statistically analyze.30 patients were randomly selected.Each patient was tested for HPV E6/E7 mRNA by two specimens(cervical exfoliated cells and cervical paraffi n-embedded tissue specimens).Results The positive rate of HPV E6/E7 mRNA increased with the severity of cervical lesions.Both exfoliated cervical cells and cervical paraffi n embedded tissue specimens could be used to detect HPV E6/E7 mRNA,The positive rate of HPV E6/E7 mRNA is not statistically signifcant.Conclusion The positive rate of HPV E6/E7 mRNA is related with the severity of cervical lesions.Both cervical exfoliated cells and cervical paraffi n embedded tissue can be used as samples to detect HPV E6/E7 mRNA in cervical cancer screening.

关 键 词：人乳头瘤病毒 宫颈病变 HPV E6/E7 MRNA 

分 类 号：R737.33[医药卫生—肿瘤]