BALB/c小鼠全子宫切除模型的建立及机制探讨  

Establishment and mechanism of a BALB/c mouse model of total hysterectomy

作　　者：常鹏环 杨泽妹[1] 林铃[1] 陈霞 林卫琼[1] 王海燕[1] 林莉香 杨照新[2] CHANG Penghuan;YANG Zemei;LIN Ling;CHEN Xia;LIN Weiqiong;WANG Haiyan;LIN Lixiang;YANG Zhaoxin(Haikou people’s Hospital,Haikou 570208,China;Hainan Medical College,Haikou 571199)

机构地区：[1]海口市人民医院妇产科,海口570208 [2]海南医学院海南省药物安评中心,海口571199

出　　处：《中国比较医学杂志》2018年第4期33-37,共5页Chinese Journal of Comparative Medicine

基　　金：海南省自然科学基金面上项目(817144)

摘　　要：目的建立BALB/c小鼠全子宫切除实验动物模型,探讨和分析该模型的特征及形成机制。方法分为空白对照组,假手术组和模型组。空白对照组不予任何处理,常规饲养;模型组经腹行全子宫切除术;假手术组仅切开皮肤,暴露子宫。术后常规饲养,动态观察各组小鼠体重变化,于4周末检测外周血雌二醇含量及卵巢组织学改变。结果术后各组实验小鼠均全部存活,一般症状良好,切口未见明显炎症,完全愈合;各组实验小鼠术前体重未见明显差异,术后第2周末、3周末和4周末,模型组小鼠体重均高于对照组和假手术组(P<0.05);模型组小鼠外周血雌二醇含量明显低于对照组和假手术组(P<0.01);空白对照组和假手术组小鼠卵巢组织学检查未见异常,模型组出现卵泡形态不规则,颗粒细胞松散、凋亡等异常组织学改变。结论该实验动物模型能够很好地模拟临床术后卵巢功能衰退等临床表现,是研究子宫切除术后并发症及筛选药物理想的动物模型。Objective To establish a BALB/c mouse model of total hysterectomy and to investigate and analyze its characteristics and formation mechanism.Methods The control group was fed conventionally without any treatment.The mice in the model group were anesthetized and performed hysterectomy.The animals in the sham-operated group had abdominal cavity opened and only exposing the uterus.The changes of body weight were observed dynamically,and at the end of the fourth weeks.The concentration of serum estradiol was determined and histological structure of the ovaries was examined.Results The general status of all the mice after operation was good without obvious inflammation,and the incision healed completely.There was no significant difference in the body weight before operation among the mice,whereas the weight of model group mice was higher than the control group and sham-operation group at the end of the second,third and forth weeks(P<0.05).The estradiol contents of the model group was significantly lower than that in control group and sham operation group(P<0.01).The histological structure of ovaries in the control group and sham-operation group was normal,however the model group showed differences,such as irregular shape of the ovaries,changed number of mature follicles,loosely distributed granulosa cells,and apoptosis.Conclusions The mouse model of total hysterectomy established in our study can well simulate the clinical manifestations of ovarian dysfunction after operation,and is an ideal model for the study of complications after ovariectomy and screening of drugs.

关 键 词：子宫切除术 卵巢 雌激素 肥胖症 动物模型 

分 类 号：R-33[医药卫生]