不同年龄段雌性Wistar大鼠不同骨骼区域骨密度值的测定与比较  

Determination and comparison of the bone mineral density of different skeletal regions of female Wistar rats at different ages

作　　者：叶尤松[1] 王陈芸 李哲丽[1] 马开利[1] 唐东红[1] YE Yousong;WANG Chenyun;LI Zheli;MA Kaili;TANG Donghong(Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Kunming 650118,China)

机构地区：[1]中国医学科学院/北京协和医学院医学生物学研究所,昆明650118

出　　处：《中国比较医学杂志》2018年第4期65-68,共4页Chinese Journal of Comparative Medicine

基　　金：中国医学科学院医学与健康科技创新工程重大协同创新项目(2016-I2M-2-006);国家自然科学基金(81571254;81570749)

摘　　要：目的提供不同年龄段雌性Wistar大鼠不同骨骼区域骨密度参考值。方法选取30只SPF级雌性Wistar大鼠,分别在6月龄、10月龄、12月龄、24月龄和30月龄时,采用双能X线骨密度仪依次测量大鼠头部、上肢、大腿、躯干、肋骨、骨盆、脊柱及全身等骨骼区域的骨密度,并进行不同年龄段间和同一年龄段不同骨骼区域间骨密度值的比较。结果随着月龄的增加雌性大鼠头部、上肢、大腿、躯干、肋骨、骨盆、脊柱和全身骨密度值迅速增加至10月龄达到峰值;大鼠头部、上肢、大腿、躯干和肋骨部骨密度值均显著或极显著高于同月龄全身骨密度值(P<0.05或P<0.01),而骨盆和脊柱部骨密度值和同月龄全身骨密度值比较差异无显著性(P>0.05),相关性分析三者均呈显著正相关(P<0.01)。结论本文提供了雌性Wistar大鼠骨生物学指标的部分背景数据,为以Wistar大鼠为对象的骨代谢研究及其在生物医学领域的应用补充了基础参考资料。Objective To provide reference values for bone mineral density(BMD)in different skeletal regions of female Wistar rats at different ages.Methods Thirty SPF female Wistar rats were selected.The BMD of different skeletal regions(skull,upper limbs,thighs,trunk,ribs,pelvis,spine and the whole body)was measured by dual energy X-ray absorptiometry(DXA)at 6,10,12,24 and 30 months of age.The bone mineral densities between different age groups and that of different skeletal regions in the same age groups were compared.Results The BMD of the skull,upper limbs,thighs,trunk,ribs,pelvis,spine and the whole body was increased rapidly with age,and reached a peak at 10 months of age.The BMD of the skull,upper limbs,thighs,trunk,ribs were significantly higher than the whole body BMD in the same month-age group(P<0.05 or P<0.01).However,there was no significant difference between the pelvic,spine and the whole body BMD(P>0.05).There was a significant positive correlation among the three correlations(P<0.01).Conclusions Some background data are provided for the bone biology studies of female Wistar rats,and provide useful supplementary reference for the studies of bone metabolism in rats and their application in biomedicine.

关 键 词：WISTAR大鼠 骨骼区域 骨密度 

分 类 号：R-33[医药卫生]