干扰E2F3基因对前列腺癌细胞侵袭、迁移的影响  

Effect of E2F3 gene interfering on the invasion and migration of prostate cancer cells

作　　者：蒋昱枫[1] 张炜[1] 李航 张璐 JIANG Yufeng;ZHANG Wei;LI Hang;ZHANG Lu(Department of Urology,The First Affiliated Hospital of Soochow University,Suzhou 215006,China)

机构地区：[1]苏州大学附属第一医院泌尿外科,江苏苏州215006

出　　处：《中国比较医学杂志》2018年第4期88-92,共5页Chinese Journal of Comparative Medicine

摘　　要：目的探究干扰转录因子E2F3基因表达对前列腺癌细胞侵袭、迁移的影响及可能的作用机制。方法转染siRNA干扰前列腺癌Du145细胞中E2F3基因表达,实验分为对照组、Du145 NC组(转染siRNA-NC)、Du145-siRNA组(转染siRNA-E2F3)。Transwell侵袭实验和划痕实验检测细胞迁移、侵袭能力,蛋白质印迹法(western blot)检测细胞中E2F3、钙黏蛋白E(E-cadherin)、基质金属蛋白酶-9(MMP-9)蛋白的表达量。结果转染siRNA-E2F3后,Du145细胞中E2F3蛋白的表达量显著低于对照组(P<0.01);Du145-siRNA组细胞的侵袭数目、划痕愈合率显著低于对照组(P<0.01);Du145-siRNA组细胞中E-cadherin蛋白表达上调(P<0.01),MMP-9蛋白表达下调(P<0.01)。结论干扰E2F3可降低前列腺癌Du145细胞的迁移和侵袭能力,该作用可能通过调控Ecadherin和MMP-9蛋白表达实现的。Objective To investigate the effect of interfering with E2F3 gene expression on the invasion and migration of prostate cancer cells and its mechanism.Methods The expression of E2F3 gene in human prostate cancer Du145 cells was knocked down by siRNA.The cells were divided into three groups:control group,Du145 NC group(siRNA-NC)and Du145-siRNA group(siRNA-E2F3).Cell migration and invasion were detected by Transwell invasion and wound healing assay.The expressions of E2F3,E-cadherin and MMP-9 proteins were detected by western blotting.Results After transfection,the expression of E2F3 protein in the Du145-siRNA group was significantly lower than the control group(P<0.01).The number of invasive cells and wound healing rate of Du145 cells in the Du145-siRNA group were significantly lower than the control group(P<0.01).Furthermore,the protein expression of E-cadherin was significantly increased(P<0.01)while MMP-9 decreased(P<0.01)in the Du145-siRNA group.Conclusions E2F3 silencing can inhibit the invasion and migration ability of prostate cancer Du145 cells,and this might be accomplished by regulating E-cadherin and MMP-9 protein.

关 键 词：E2F3 前列腺癌 侵袭 迁移 E-CADHERIN 

分 类 号：R-33[医药卫生]