体外染色体畸变试验染色体标本制备的质量控制  

Quality control of chromosome preparation in the chromosome aberration test in vitro

作　　者：贾庆文 英永 马会 朱春花 高梅 JIA Qingwen;YING Yong;MA Hui;ZHU Chunhua;GAO Mei(Shandong Academy of Pharmaceutical Sciences,Shandong Provincial Key Laboratory of Chemical Drugs,Jinan 250101,China;Shandong Normal University,College of Life Sciences,Shandong Provincial Key Laboratory of Animal Resistance Biology,Jinan 250014)

机构地区：[1]山东省药学科学院,山东省化学药物重点实验室,济南250101 [2]山东师范大学生命科学学院,山东省动物抗性生物学重点实验室,济南250014

出　　处：《中国比较医学杂志》2018年第4期108-112,共5页Chinese Journal of Comparative Medicine

基　　金：山东省创新公共服务平台项目(2015CXPT00001)。

摘　　要：目的探讨体外染色体畸变试验染色体制备过程的影响因素及质量控制,总结分析体外染色体标本成功制备方法及要点。方法选用中国仓鼠肺细胞(CHL)进行细胞培养及染色体制备,阳性诱变剂选用丝裂霉素和环磷酰胺,经过常规低渗、固定、滴片,最后镜检阅片。结果 CHL染色体畸变试验,丝裂霉素、环磷酰胺处理后染色体畸变率显著增高,均>20%;而阴性对照组在有和无代谢活化系统两种测试条件下,染色体结构畸变率均小于5%。所制备的染色体分散良好,长短适中,成功率较高。结论影响体外染色体畸变试验细胞染色体标本制备的因素很多,每一个步骤都很关键,必须严格按照操作规程,掌握每一个步骤的原理,细致、耐心、科学地操作,才能制备出好的标本。Objective To study the influencing factors and quality control of chromosome preparation during chromosome aberration test in vitro,and to summarize and analyze the method and key points of successful preparation of chromosome specimens in vitro.Methods Chinese hamster lung cells(CHL)were used for cell culture and chromosome preparation.Mitomycin and cyclophosphamide were used as positive mutagens.After routine hypotonic treatment,fixation,and squash preparation,finally,to read the film under the microscope.Results The CHL chromosome aberration test showed that both the chromosome aberration rates of mitomycin-and cyclophosphamide-treated cells were significantly increased(>20%),while the aberration rates in the negative control group were less than 5%,either with and without metabolic activation.The success rate was high and the prepared chromosomes were well dispersed with a moderate length.Conclusions Many factors can affect the specimen preparation in chromosome aberration test.Every step is very important,and it should be strictly following the operating procedures.It is of importance to grasp the principle of each step and to operate carefully,patiently and scientifically in order to prepare good specimens.

关 键 词：染色体畸变试验 染色体标本制备 质量控制 中国仓鼠肺细胞 

分 类 号：R-33[医药卫生]