HIV-1感染者CD73+CD8+T细胞减少与T细胞异常活化的相关性研究  

Correlation between decreased CD73^+CD8^+ T cells and T-cell abnormal activation in HIV-1 infected patients

作　　者：常文仙 张和倩 周双男[2] 黄辉煌[2] 涂波[2] 周春保 张纪元[2] 王福生 CHANG Wen-xian;ZHANG He-qian;ZHOU Shuang-nan;HUANG Hui-huang;TU Bo;ZHOU Chun-bao;ZHANG Ji-yuan;WANG Fu-sheng(Department of Clinical Medicine,BengBu Medical College,233000,China)

机构地区：[1]蚌埠医学院临床医学系,233000 [2]解放军第三〇二医院感染病诊疗与研究中心,北京100039

出　　处：《传染病信息》2018年第2期145-148,160,共5页Infectious Disease Information

基　　金：首都特色基金(Z161100000516011)

摘　　要：目的探讨HIV-1感染者外周血CD8^+T细胞上CD73的表达特点及其与T细胞异常活化和疾病进展的关系。方法研究入选65例HIV-1感染者和27例健康对照。通过流式细胞术检测研究对象外周血CD73^+CD8^+T细胞的频率和绝对计数,并将患者CD73^+CD8^+T细胞绝对计数和频率与其CD4^+T细胞计数、HIV-1载量以及CD38^+CD8^+T细胞频率进行相关性分析。结果与健康对照相比,HIV-1感染者外周血CD73^+CD8^+T细胞绝对计数和频率均明显降低(P均<0.05);HIV-1感染者外周血CD73^+CD8^+T细胞绝对计数和频率与CD4^+T细胞计数呈正相关(r=0.555,P=0.001;r=0.342,P=0.005),与CD38^+CD8^+T细胞频率呈负相关(r=-0.384,P=0.002;r=-0.387,P=0.001);HIV-1感染者CD73^+CD8^+T细胞的绝对计数与HIV-1载量呈弱负相关(r=-0.261,P=0.035)。结论 HIV-1感染者外周血CD73^+CD8^+T细胞的减少不但与患者T细胞的活化程度呈显著负相关,而且与AIDS疾病进展相关。Objective To detect the expression of CD73 on peripheral blood CD8+T cells in HIV-1 infected patients and analyze its correlation with disease progression and T cell abnormal activation.Methods Twenty-seven health controls and 65 HIV-1 infected patients were enrolled in this study.The frequency and absolute count of peripheral blood CD73+CD8+T cells in all subjects were detected by flow cytometry and its correlation with CD4+T cell counts,HIV-1 viral load and frequency of CD38+CD8+T cells were analyzed.Results Compared with healthy controls,the absolute count and frequency of CD73+CD8+T cells were significantly reduced in peripheral blood of HIV-1 infected patients(P<0.05);the absolute count and frequency of CD73+CD8+T cells in peripheral blood of HIV-1 infected patients were positively correlated with CD4+T cell counts(r=0.555,P=0.001;r=0.342,P=0.005),and negatively correlated with frequency of CD38+CD8+T cells(r=-0.384,P=0.002;r=-0.387,P=0.001);there was a negative correlation between the absolute count of CD73+CD8+T cells and the HIV-1 viral load in HIV-1 infected patients(r=-0.261,P=0.035).Conclusions The decrease of CD73+CD8+T cells in HIV-1 infected patients is not only significantly negatively correlated with T cell activation,but also associated with progression of AIDS.

关 键 词：艾滋病 CD73 T细胞活化 疾病进展 

分 类 号：R512.91[医药卫生—内科学]