早期应用清热化痰解毒汤对实验性缺血性脑卒中大鼠缺损神经的影响  

Effects of early application of Qingrehuatanjiedu decoction on nerve defects in rats with experimental ischemic stroke

作　　者：王涛[1] 刘宏祥[1] 王颖[1] 齐姣[1] 史小盼[1] 陈小静[1] 闫丽静 王珍[1] WANG Tao;LIU Hongxiang;WANG Ying(Department of Integrated Traditonal Chinese and Western Medicine,Affiliated Hospital of Hebei University,Hebei,Baoding 071000,China)

机构地区：[1]河北大学附属医院中西医结合科,河北省保定市071000

出　　处：《河北医药》2018年第9期1311-1314,1318,共5页Hebei Medical Journal

基　　金：河北省科学技术研究与发展支撑计划项目(编号:132777203)

摘　　要：目的探讨清热化痰解毒汤对实验性缺血性脑卒中大鼠缺损神经的影响。方法采取双侧颈动脉结扎,并给予硝普钠腹腔注射制作SD大鼠模型,分为假手术组、缺血模型组、中药治疗组(即清热化痰解毒汤)、尼莫地平组,采用干湿法测脑组织含水量及HE染色观察脑组织形态学变化,并观察大鼠神经功能缺失评分的变化。结果与假手术组比较,缺血模型组在缺血24 h内脑组织的含水量开始缓慢增加(P<0.05),但与中药治疗组、尼莫地平组比较差异无统计学意义(P>0.05),而到缺血72 h时缺血模型组大鼠脑组织含水量则有明显增加,与24 h时比较差异有统计学意义(P<0.05);中药治疗组应用清热化痰解毒汤后实验大鼠脑组织含水量明显下降,其中在缺血72 h时间点的脑组织含水量与模型组比较差异有统计学意义(P<0.05);同时尼莫地平组在缺血72 h时实验大鼠脑组织的含水量较缺血模型组明显下降,差异有统计学意义(P<0.05)。72 h后中药治疗组和尼莫地平组大鼠脑组织毛细血管周围间质水肿明显减轻。同时大鼠脑组织形态学及神经缺失评分均有改善。结论清热化痰解毒汤能够减轻急性缺血性脑卒中大鼠脑水肿,改善其脑神经功能。Objective To investigate the effects of early application of Qingrehuatanjiedu decoction on nerve defects in rats with experimental ischemic stroke.Methods The SD rat models with ischemic stroke were established by bilateral carotid ligation and intraperitoneal injection of sodium nitroprusside,then these rats were divided into sham operation group,ischemic model group,traditional Chinese medicine treatment group(Qingrehuatanjiedu decoction,TCM group),nimodipine control group.The dry and wet method was used to measure the water content of brain tissue,and HE staining was used to observe the morphological changes of brain tissues and the changes of nerve dysfunction scores in rats.Results As compared with that in sham operation group,the water content of cerebral tissues in ischemic model group was increased slowly in 24h after cerebral ischemia(P<0.05),however,there was no significant difference with that inTCM group and nimodipine group(P>0.05).However the water content of cerebral tissues in ischemic model group was increased significantly at 72h after cerebral ischemia,which was statistically different from that in 24h(P<0.05).After treatment with Qingrehuatanjiedu decoction,the water content of cerebral tissues in TCM group was significantly decreased,there was significant difference at 72h after cerebral ischemia between TCM group and ischemic model group(P<0.05),at the same time,the water content of cerebral tissue in nimodipine control group was significantly lower than that in ischemic model group(P<0.05).The interstitial edema around the capillaries of cerebral tissues at 72h after cerebral ischemia in TCM group and nimodipine control group was significantly relieved,meanwhile,nerve dysfunction scores were obviously improved in the two groups.Conclusion Qingrehuatanjiedu decoction can relieve brain edema in rats with acute ischemic stroke and improve cranial nerve function of rats.

关 键 词：清热化痰解毒汤 缺血性脑卒中 大鼠脑组织含水量 神经缺失评分 

分 类 号：R745.1[医药卫生—神经病学与精神病学]