氯化两面针碱通过细胞色素c介导的线粒体凋亡途径诱导人结肠癌HT29细胞凋亡  

Nitidine chloride induced colorectal cancer HT29 cells apoptosis through the cytochrome c-mediated mitochondrial apoptotic pathways

作　　者：黄燚燚 秦悦[1] 谢雪平[1] 傅宣皓 黄丹 刘华钢[1] 赖泽锋[1] Huang Yiyi;Qin Yue;Xie Xueping;Fu Xuanhao;Huang Dan;Liu Huagang;Lai Zefeng(Pharmaceutical College,Guangxi Medical University,Nanning 530021,China;Basic Medical College,Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学药学院,南宁530021 [2]广西医科大学基础医学院,南宁530021

出　　处：《广西医科大学学报》2018年第4期426-430,共5页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.81260657)

摘　　要：目的:初步探讨氯化两面针碱(NC)对人结肠癌HT29细胞凋亡的影响及其机制。方法:将HT29细胞分为溶剂对照组、15μmol/L NC组、30μmol/L NC组和60μmol/L NC组,采用CCK-8法检测NC对细胞增殖的抑制作用,流式细胞术检测细胞凋亡,western blotting法检测细胞色素c(Cyt-c)和caspase-3蛋白的表达水平。结果:与对照组相比,各NC实验组的细胞存活率显著降低,凋亡率显著升高,15μmol/L NC组、30μmol/L NC组和60μmol/L NC组细胞凋亡率分别为(35.30±14.30)%、(41.48±17.89)%和(46.51±11.35)%,差异具有统计学意义(P<0.05)。与对照组比较,不同浓度NC组Cyt-c和caspase-3蛋白表达明显上调(P<0.05)。结论:NC能够显著抑制人结肠癌HT29细胞的增殖,诱导HT29细胞凋亡,其分子机制可能与Cyt-c介导的线粒体凋亡途径有关。Objective:To preliminarily explore the effect of nitidine chloride(NC)on apoptosis of HT29 colorectal cancer cell line and the related mechanism.Methods:HT29 cells were divided into vehicle control group and NC groups(15,30 and 60μmol/L).CCK-8 assay and flow cytometry were used to detect cell proliferation and apoptosis,respectively.Western blotting was employed to determine the protein levels of cytochrome-c(Cyt-c)and caspase-3.Results:Compared with the vehicle control group,the cell viability rates of the NC groups decreased obviously and the apoptosis rates[(35.30±14.30)%,(41.48±17.89)%and(46.51±11.35)%]increased prominently,and differences were statistically significant(P<0.05).Western blot showed that the protein levels of Cyt-c and caspase-3 in the NC groups were notably upregulated compared with the vehicle control group(P<0.05).Conclusion:NC could significantly inhibit the proliferation of HT29 cells and induce apoptosis,and the mechanism might be associated with the Cyt-c mediated mitochondrial apoptotic pathways.

关 键 词：氯化两面针碱 人结肠癌 线粒体凋亡途径 CYT-C CASPASE-3 

分 类 号：R735.35[医药卫生—肿瘤]