Vimentin和Ki-67在肾癌中的表达及其临床意义  

Clinical significance of Vimentin and Ki-67 expression in renal cell carcinoma

作　　者：刘德乾[1] 赵战魁[2] 孟琳[2] 张雪 陈晔[2] Liu Deqian;Zhao Zhankui;Meng Lin;Zhang Xue;Chen Ye(Jining Medical University,Jining,272067;Department of Urology,the Affiliated Hospital of Jining Medical University,Jinning,272029,China)

机构地区：[1]济宁医学院,在读研究生272067 [2]济宁医学院附属医院泌尿外科,272029

出　　处：《中华诊断学电子杂志》2018年第2期134-138,共5页Chinese Journal of Diagnostics(Electronic Edition)

基　　金：国家自然科学基金(8150041576)

摘　　要：目的观察Vimentin和Ki-67在肾癌(RCC)组织中的表达,探讨Vimentin和Ki-67在RCC发生、发展中的作用及其相互关系。方法收集2016年1月至2017年6月在济宁医学院附属医院泌尿外科行手术治疗的133例RCC患者的临床资料。应用免疫组化方法(SP法)检测不同组织中Vimentin和Ki-67的表达情况,并与肿瘤临床分期、组织学分级及肿瘤大小进行相关性分析。结果Vimentin及Ki-67在RCC组织中均异常高表达,在癌旁正常肾组织中均不表达,差异具有统计学意义(χ~2=58.86,P<0.05;χ~2=26.67,P<0.05);Vimentin和Ki-67的表达在不同RCC患者性别(χ~2=4.07,1.16;P>0.05)、年龄(χ~2=0.03,0.87;P>0.05)、肿瘤位置(χ~2=0.00,0.00;P>0.05)之间的差异无统计学意义,在RCC的临床分期(χ~2=10.66,P<0.01;χ~2=19.88,P<0.01)、组织学分级(χ~2=13.79,P<0.01;χ~2=21.03,P<0.01)、肿瘤的大小(χ~2=19.52,P<0.01;χ~2=16.10,P<0.01)差异具有统计学意义,两者的表达呈正相关(r=0.46,P<0.05)。结论 Vimentin和Ki-67参与了RCC的发生发展,其表达水平与组织学分级、临床分期、肿瘤大小有关,组织学分级越高、临床分期越晚、肿瘤体积越大,两者的表达水平越高,两者在肿瘤的进展中可能起到协同作用。Objective To observe the expression levels of Vimentin and Ki-67 in the occurrence and development of renal cell carcinoma(RCC)and explore the possible correlation between the expression of Vimentin/Ki-67 and clinical significance.Methods The clinical data of 133 patients who underwent surgical operation from January 2016 to June 2017 were reviewed.SP immunohistochemical staining was used to explore the expression levels of Vimentin and Ki-67 in different tissues.Correlation analysis was carried out between the expression results and the clinical stage,histological grade and tumor size.Results Both Vimentin and Ki-67 showed positive expression in renal cell carcinoma,but no expression was found in the adjacent normal renal tissue.Vimentin was mainly expressed in the cytoplasm of the cells while Ki-67 expressed in nucleus.There was significant difference between renal cell carcinoma and adjacent normal renal tissue(χ2=58.86,P<0.05;χ2=26.67,P<0.05).There were no significant differences between Vimentin/Ki-67 expression levels in different gender(χ2=4.07,P>0.05;χ2=1.16,P>0.05),age(χ2=0.03,P>0.05;χ2=0.87,P>0.05)and location(χ2=0.00,P>0.05;χ2=0.00,P>0.05)of renal cell carcinoma patients.However,there were significant differences between different clinical stage(χ2=10.66,P<0.01;χ2=19.88,P<0.01),histological grade(χ2=13.79,P<0.01;χ2=21.03,P<0.01)and tumor size(χ2=19.52,P<0.01;χ2=16.10,P<0.01).There was positive correlation between Vimentin/Ki-67(r=0.46,P<0.05).Conclusions Vimentin and Ki-67 are involved in the development of renal cell carcinoma,and their expression levels are related to the clinical stage,histological grade and tumor size.High expression levels of Vimentin and Ki-67 are presented in carcinoma tissues with high clinical stage,late histological grade and big tumor.Both of them may play a synergistic role in the progression of the carcinoma.

关 键 词：肾肿瘤 波形蛋白 KI-67 免疫组织化学 

分 类 号：R737.11[医药卫生—肿瘤]