结肠癌组织中STAT3和Survivin蛋白的表达及与临床病理特征的关系  

Expression and Clinical Significance of Signal Transducers and Activator of Transcription 3 and Survivin Protein in Colon Cancer

作　　者：孟玮[1] 史晓宇[1] 刘博[2] 张敬[1] 马峰[1] 赵峻峰[1] 孙守燕[1] 王世旭[1] MENG Wei;SHI Xiaoyu;LIU Bo;ZHANG Jing;MA Feng;ZHAO Junfeng;SUN Shouyan;WANG Shixu(Department of Medical Oncology,Hebei North Univer-sity,Zhangjiakou 075000,China;Department of Pathology,the First Affiliated Hospital,Hebei North Univer-sity,Zhangjiakou 075000,China)

机构地区：[1]河北北方学院附属第一医院肿瘤内科,河北张家口075000 [2]河北北方学院附属第一医院病理科,河北张家口075000

出　　处：《医学综述》2018年第8期1643-1647,共5页Medical Recapitulate

基　　金：张家口市科技计划自筹经费项目(1521104D)

摘　　要：目的检测不同结肠组织中信号转导转录激活因子3(STAT3)和存活素(Survivin)蛋白的表达及与临床病理特征的关系。方法选择2015年6月至2017年6月河北北方学院附属第一医院收治的结肠癌切除标本40例为研究对象,另取同期手术切除非结肠癌的正常结肠组织20例作为对照,应用免疫组织化学法检测两组STAT3和Survivin的表达情况,同时采用逆转录实时定量聚合酶链反应的方法检测两组中STAT3的相对表达量。结果在结肠癌组织中STAT3和Survivin蛋白表达阳性表达率分别为80.0%(32/40)、77.5%(31/40),明显高于正常结肠组织中两者的阳性表达率[20%(4/20)、25%(5/20)](P<0.01)。低+未分化、Ⅲ+Ⅳ期、合并淋巴结转移组的STAT3和Survivin蛋白阳性表达率高于高+中分化、Ⅰ+Ⅱ期、无淋巴结转移组(P<0.05)。结肠癌组织STAT3mRNA相对表达量高于正常结肠组织(1.108±0.293比0.714±0.258,P<0.05)。相关性分析结果显示,结肠癌中STAT3与Survivin蛋白表达呈正相关(r=0.307,P<0.01)。结论 STAT3和Survivin在结肠癌中高表达,两者可能共同参与结肠癌的发生、发展,STAT3和Survivin的高表达提示预后不良。Objective To explore the expression of signal transducers and activator of transcription factor 3(STAT3)and significance of survivin protein in different colon tissues,and analyze the relationship between the expression of the two and the clinicopathologic features of colon cancer.Methods A total of 40 resected specimens of colon cancer in the First Affiliated Hospital,Hebei North University from Jun.2015 to Jun.2017 were included as the research objects,and 20 normal colon tissues without colon cancer were included as controls.Immunohistochemical method was adopted to detect the expression of STAT3 and survivin protein in the 40 colonic cancer tissues and 20 normal colonic tissues.Reverse transcription real-time polymerase chain reaction was used to detect the relative expression quantity of STAT3 in 40 colonic cancer tissues and 20 normal colonic tissues.Results The expression rate of STAT3 and survivin protein in colon cancer tissues was 80.0%(32/40)and 77.5%(31/40)respectively,the positive expression rate was significantly higher than that in the normal colonic tissues[20%(4/20),25%(5/20)](P<0.01).The positive expression rate of STAT3 and survivin protein in poorly differentiated and undifferentiated cases,stageⅢand stageⅣcases,and cases with lymph node metastasis was higher than that in cases of high and middle differentiation,stageⅠand stageⅡ,and no lymph node metastasis(P<0.05).The relative expression of STAT3 mRNA was higher than that of the normal colonic tissues(1.108±0.293 vs 0.714±0.258,P<0.05).Correlation analysis showed that the expression of STAT3 and survivin protein in colon cancer was positively correlated(r=0.307,P<0.01).Conclusion STAT3 and survivin protein are overexpresses in colonic cancer tissu e,indicating that they may be involved in the genesis and development of colon cancer,which is a predictor of poor prognosis.

关 键 词：信号转导转录激活因子3 存活素蛋白 结肠癌 免疫组织化学 逆转录实时定量聚合酶链反应 

分 类 号：R735[医药卫生—肿瘤]