长链非编码RNA SBF2-AS1在肺癌组织中的表达及作用研究  

Expression and effect of long non-coding RNA SBF2-AS1 in lung cancer tissue and non-small cell lung cancer

作　　者：杜玮[1] 周建龙[1] 叶丹 Wei Du;Jian-long Zhou;Dan Ye(The 6th Department of Internal Medicine,Guangzhou General Hospital of Guangzhou Military Command,Guangzhou,Guangdong 510010,China;Hexian Memorial Hospital,Panyu,Guangzhou,Cuangdong 511400,China)

机构地区：[1]广州军区广州总医院内六科,广东广州510010 [2]广州市番禺区何贤纪念医院,广东广州511400

出　　处：《中国现代医学杂志》2018年第13期40-44,共5页China Journal of Modern Medicine

基　　金：广东省医学科学技术研究基金(No:A2015240)

摘　　要：目的探讨长链非编码RNA SBF2-AS1在肺癌组织中的表达及对非小细胞肺癌细胞系A549及H1299细胞增殖、凋亡等的影响。方法 51例肺癌组织标本(包括癌组织及癌旁组织),实时荧光定量聚合酶链反应检测SBF2-AS1的表达量,分析其与临床病例特征的关系;用siRNA干扰A549及H1299细胞SBF2-AS1的表达,MTT及流式细胞术分别检测其对细胞增殖、凋亡及周期的影响。结果 SBF2-AS1在癌组织中的表达量是癌旁组织的5.05倍;且其表达水平与淋巴结转移、临床病理分期有密切关系。A549及H1299细胞中SBF2-AS1的表达量被si RNA干扰下降后,细胞的增殖减慢,凋亡增加,细胞周期被阻滞于G1/G0期。结论SBF2-AS1在肺癌组织中高表达,可成为新的潜在的肺癌预测、预后判断的分子标志物及治疗靶点。Objective To investigate the expression of long non-coding RNA SBF2-AS1 in lung cancer tissues and its effect on growth and apoptosis of non-small cell lung cancer(NSCLC).Methods qRT-PCR was used to test the expression of SBF2-AS1 in 51 lung cancer tissues and matched adjacent normal tissues.Its correlations with the clinical parameters were analyzed.siRNA was used to knock down SBF2-AS1 expression in A549 and H1299 cells,its roles in cell growth and apoptosis were investigated by MTT and flow cytometry.Results The expression of SBF2-AS1 in the lung cancer tissues was significantly upregulated as compared with the matched adjacent normal tissues.Increased SBF2-AS1 expression was significantly correlated with tumor stage and lymph node metastasis.Knocking down SBF2-AS1 significantly reduced cell growth,promoted cell apoptosis,and resulted in cell cycle arrest at G1/G0 phase.Conclusions High expression of SBF2-AS1 is involved in lung cancer progression and SBF2-AS1 may be a potential target for diagnosis of NSCLC and gene therapy.

关 键 词：长链非编码RNA SBF2-AS1 肺癌 增殖 凋亡 周期 

分 类 号：R734.2[医药卫生—肿瘤]