大鼠球囊巩膜外加压后眼压和虹膜血管的改变  

Longitudinal changes in intraocular pressure and iris vessels in rats with bulb external scleral explants

作　　者：刘克高[1] 章征[1] 颜繁城 LIU Ke-Gao;ZHANG Zheng;YAN Fan-Cheng(From the Department of Ophthalmology,Beijing Tongren Hospital,Affiliated Hospital of Capital Medical University,Beijing Ophthalmology&Visual Sciences Key Lab,Beijing 100730,China;Beijing Tongren Eye Center,Capital Medical University,Beijing Institute of Ophthalmology,Beijing 100730,China)

机构地区：[1]首都医科大学附属北京同仁医院眼科,北京市眼科学与视觉科学重点实验室,北京市100730 [2]首都医科大学附属北京同仁眼科中心,北京市眼科研究所,北京市100730

出　　处：《眼科新进展》2018年第5期421-424,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:81600725)

摘　　要：目的探讨巩膜外加压对大鼠加压后即刻和不同时段眼压(intraocular pressure,IOP)、角膜和虹膜血管的影响。方法取正常大鼠18只。于每只大鼠右眼切开结膜,在外直肌下方放置球囊导管,充气加压4～5 ATM(1 ATM=101.325 k Pa)。于加压、撤压前后不同时段分别测量大鼠的IOP,拍摄眼前节像。观察IOP、角膜和虹膜血管的改变。结果加压后即刻IOP(40.66±10.55)mmHg(1 kPa=7.5 mmHg)迅速高出基线(8.45±1.23)mmHg(P=0.000)。加压后15 min、30 min、45 min、60 min后IOP逐渐下降,但仍高出基线水平,除60 min(P=0.929)外,其他3个时间点与基线水平差异均有统计学意义(均为P<0.05)。撤除巩膜外加压后即刻的IOP(6.09±0.49)mmHg迅速低于基线水平(P=0.000)。撤压后15 min、30 min后IOP逐渐恢复到基线水平。巩膜外加压后角膜水肿、虹膜血管扩张。撤除巩膜外加压后角膜出现皱褶,虹膜血管仍然扩张。结论球囊巩膜外加压后即刻IOP迅速增高,以后逐渐下降。随着加压时间的延长对侧眼的IOP有逐渐增高的趋势。球囊巩膜外加压可能影响大鼠的葡萄膜巩膜房水流出途径。Objective To observe longitudinal changes in the intraocular pressure(IOP),the cornea and iris blood vessels in normal rats with bulb external scleral explants.Methods Totally 18 normal rats were enrolled in the experiment.Conjunctival incisions of the right eyes of these rats were performed,followed by the placement of a balloon catheter under the lateral rectus muscle and inflation at 4-5 ATM.The IOP was measured and iris images were taken longitudinally before and after pressuring and pressure withdrawing respectively.Results IOP was elevated to(40.66±10.55)mmHg(1 kPa=7.5 mmHg)immediately after pressing,which was over the baseline(8.45±1.23)mmHg(P=0.000),and then decreased gradually at 15 min,30 min,45 min,60 min after pressing,but the IOPs were still greater than the baseline.There were significant differences in IOPs at 15 min,30 min,45 min after pressing and the baseline(all P<0.05),while no difference in this variable at 60 min after pressing and the baseline(P=0.929).However,the IOP was quickly reduced to(6.09±0.49)mmHg at once after pressure withdrawing,which was below the baseline(P=0.000),and then gradually returned to the baseline at 15 min,30 min after pressure withdrawing.The corneal edema and dilation of iris vessels were observed after pressuring,and following corneal winkle and dilated iris vessels after pressure withdrawing.Conclusion Bulb external scleral explant can result in elevated IOP immediately,then decreased gradually.With the increase of compressing,the IOP of the contralateral eye tends to increase.Bulb external scleral explant may affect uveoscleral outflow pathway of aqueous humor in rats.

关 键 词：大鼠 球囊导管 巩膜外加压 眼压 虹膜血管 

分 类 号：R775[医药卫生—眼科]