黑龙江省椴树蜂蜜体外抑制金黄色葡萄球菌的实验研究  

Study on inhibition of Staphylococcus aureus in vitro of linden honey in Heilongjiang Province

作　　者：熊亚敏 张伟滨 罗洋 陈炳坤 吴文忠 姜波[1] 张锦明[1] XIONG Ya-min;ZHANG Wei-bin;LUO Yang;CHEN Bing-kun;WU Wen-zhong;JIANG Bo;ZHANG Jin-ming(the First Affiliated Hospital of Harbin Medical University,Heilongjiang Harbin,150001,China;Heilongjiang Province Yingchunlin area the People's Hospital,Heilongjiang Jixi 158100,China)

机构地区：[1]哈尔滨医科大学附属第一医学院,黑龙江哈尔滨150001 [2]黑龙江省迎春林区人民医院,黑龙江鸡西158100

出　　处：《临床医药文献电子杂志》2017年第60期11688-11690,11880,共4页Electronic Journal of Clinical Medical Literature

基　　金：国家临床重点专科建设项目经费资助(2012-649)

摘　　要：目的研究黑龙江省不同地区椴树蜜对金黄色葡萄球菌的体外抗菌作用。方法采用微孔板改良法复制金黄色葡萄球菌模型,将蜂蜜与无菌的培养基(Mueller-Hinton Broth培养基)混合后得到5%~50%(w/v)的不同浓度,用不加蜂蜜的无菌培养基和麦卢卡蜂蜜作对照,测定蜂蜜的最低抑菌浓度(MIC)及最低杀菌浓度(MBC)。结果各品种蜂蜜均有抗菌性及杀菌性,但其抗菌及杀菌作用各有不同,其中以迎春椴树黑蜂蜜(MIC为25%,MBC为25%)的抗菌作用较好,相当于麦卢卡15+,其余各类型椴树蜜均具有不同程度的抗菌性作用。结论椴树蜜对于金黄色葡萄球菌有抑菌性和杀菌性,不同地域的椴树蜜对同一细菌具有不同的抗菌活性,其中以迎春地区黑蜂椴树蜜为最佳,为开发和利用蜂蜜作为临床抗菌药物提供了一定的依据。Objective The antibacterial effect of linden honey in different areas of Heilongjiang Province on Staphylococcus aureus in vitro.Methods By using the improved method of replication of Staphylococcus aureus model,honey and sterile culture medium(Mueller-Hinton Broth medium)mixed 5%~50%(w/v)of different concentrations,compared with sterile culture medium without honey and honey,the minimum inhibitory concentration and minimum bactericidal concentration MIC MBC determination of honey.Results All kinds of honey are antibacterial and bactericidal,but its antibacterial and bactericidal effect are different,of which the spring ting tree black honey(MIC 25%,MBC 25%)of the antibacterial effect is better,the equivalent of medical grade honey Manuka UMF15+,the rest of the various types of linden tree honey have different degrees of antibacterial effect.Conclusion Linden honey had antimicrobial properties and sterilization to Staphylococcus aureus,different regions have different antibacterial activity of linden honey on the same bacteria,of which spring area black linden honey bee is the best for the development and utilization of honey as clinical antibacterial drugs provided certain basis.

关 键 词：蜂蜜 最低抑菌浓度 最低杀菌浓度 金黄色葡萄球菌 

分 类 号：R378.11[医药卫生—病原生物学]