利用脂肪酶水解生活污油制备脂肪酸  

Preparation of Fatty Acid from Life Waste Oil Hydrolyzed by Lipase

作　　者：张春兰[1] 陈淑芬[1] 颉林[1] 甘黎明[1] ZHANG Chun-lan;CHEN Shu-fen;XIE Lin;GAN Li-ming(Petrochemical School,Lanzhou Petrochemical Polytechnic,Lanzhou 730060,China)

机构地区：[1]兰州石化职业技术学院石油化学工程学院,甘肃兰州730060

出　　处：《兰州石化职业技术学院学报》2018年第2期1-4,共4页Journal of Lanzhou Petrochemical Polytechnic

基　　金：甘肃省高等学校科研项目(2015B-151)

摘　　要：利用脂肪酶水解生活污油制备脂肪酸,考察了单因素影响脂肪酶水解生活污油水解率的因素,并通过正交试验设计对脂肪酶水解生活污油的工艺条件进行优化,确定了最佳酶催化水解条件是:酶用量0.1 mL、pH为7、反应温度50℃、油水质量比1:2.5、水解时间9 h、摇床速度140 r/min。且在最佳条件下,脂肪酶水解生活污油的水解率为70.98%。Fatty acid was prepared from life waste oil with lipase.The factors affecting the hydrolysis rate of the life waste oil were studied through single factor experiments,and technological conditions of lipase hydrolysis were optimized by orthogonal test design.Thus,the optimum enzyme-catalyzed hydrolysis conditions were determined as follows:enzyme dosage 0.1 mL,pH 7,reaction temperature 50℃,mass ratio of oil and water 1∶2.5,hydrolysis time 9h and shaking speed 140 r/min.Under best conditions,lipase hydrolyzed life waste oil is with hydrolysis rate of 70.98%.

关 键 词：脂肪酶 生活污油 脂肪酸 水解率 

分 类 号：TQ225[化学工程—有机化工]