MiR-9对人胃癌细胞增殖和迁移的影响  

Effect of MiR-9 on Proliferation and Migration of Human Gastric Cancer Cells

作　　者：刘文明[1] 范燕云[1] 田钊旭 万曼 周飞[1] 胡益群[1] LIU Wenming;FAN Yanyun;TIAN Zhaoxu;WAN Man;ZHOU Fei;HU Yiqun(Department of Gastroenterology,Zhongshan Hospital,Xiamen University;Xiamen Digestive Disease Center,Xiamen,Fujian Province(361004))

机构地区：[1]厦门大学附属中山医院消化内科,厦门大学消化疾病研究所,厦门市消化疾病诊治中心,361004

出　　处：《胃肠病学》2018年第6期342-346,共5页Chinese Journal of Gastroenterology

基　　金：福建省卫生计生委青年科研课题(2015-2-48);厦门市科技计划指导性项目(3502Z20149006)

摘　　要：背景:MicroRNA-9(miR-9)参与调控生长、发育、细胞自我更新和多向分化等多种生理过程,而且其表达异常与多种恶性肿瘤密切相关。目的:探讨miR-9对人胃癌细胞生物学行为的影响。方法:以real-time PCR检测人正常胃黏膜上皮细胞株GES-1和人胃癌细胞株BGC-823、SGC-7901中的miR-9表达。采用瞬时转染法将miR-9类似物、miR-9抑制物和空载体分别转染BGC-823、SGC-7901细胞,并以real-time PCR验证转染效率。CCK-8实验检测细胞增殖能力,Transwell细胞迁移实验和划痕实验检测细胞迁移能力。结果:与正常胃黏膜上皮细胞相比,胃癌细胞miR-9表达显著升高(P<0.05)。与空载体对照组相比,miR-9类似物组BGC-823、SGC-7901细胞miR-9表达上调,细胞增殖和迁移能力明显增强(P<0.05),miR-9抑制物则可显著抑制胃癌细胞的增殖和迁移能力(P<0.05)。结论:上调miR-9表达可促进胃癌细胞增殖和迁移,提示miR-9过表达可能与胃癌进展有关。Background:MicroRNA-9(miR-9)is involved in the modulation of a variety of physiological process such as organ development and self-renewal and multipotential differentiation of cells.Moreover,its aberrant expression is closely associated with several types of malignant tumors.Aims:To investigate the effect of miR-9 on biological behavior of human gastric cancer cells.Methods:Expression of miR-9 in normal human gastric epithelial cell line GES-1 and human gastric cancer cell line BGC-823 and SGC-7901 was detected by real-time PCR.Then the two gastric cancer cell lines were transiently transfected with miR-9 mimic,miR-9 inhibitor and empty vector,respectively;the efficiency of transfection was assessed by real-time PCR.CCK-8 assay,Transwell assay and wound healing assay were used to examine the cell proliferation and migration capacities.Results:The expression level of miR-9 was significantly higher in gastric cancer cells than in normal gastric epithelial cells(P<0.05).Compared with cells transfected with empty vector,overexpression of miR-9 occurred in cells transfected with miR-9 mimic and effectively promoted the proliferation and migration of BGC-823 and SGC-7901 cells(P<0.05).Conversely,transfection with miR-9 inhibitor significantly suppressed the proliferation and migration of gastric cancer cells(P<0.05).Conclusions:Up-regulating miR-9 expression promotes the proliferation and migration of gastric cancer cells,which indicates that miR-9 overexpression may be associated with progression of gastric cancer.

关 键 词：胃肿瘤 微RNA9 细胞增殖 细胞迁移 

分 类 号：R735.2[医药卫生—肿瘤]