胆管细胞癌组织S100A6 mRNA及其蛋白表达变化  

Relative levels of S100A6 m RNA and its protein expression in tumorous tissues of patients with cholangiocarcinoma

作　　者：李福荣[1] 牛福勇[1] 李华[2] Li Furong;Niu Fuyong;Li Hua(Department of Hepatobiliary Surgery,First Hospital,Yulin 719000,Shaanxi Province)

机构地区：[1]陕西省榆林市第一医院肝胆外科,719000 [2]西安交通大学医学院第二附属医院

出　　处：《实用肝脏病杂志》2018年第4期541-544,共4页Journal of Practical Hepatology

基　　金：陕西省自然科学基础研究计划项目(编号:2012JQ4016);西安交通大学基础科研资助项目(编号:Xjj2012072)

摘　　要：目的检测胆管癌组织S100A6 m RNA及其蛋白表达的变化。方法在40例胆管癌患者和40例胆道良性疾病,分别取胆管癌组织、癌旁组织和肝组织,检测S100A6 m RNA及其蛋白表达变化,采用ELISA法测定血清S100A6水平。应用单因素和多因素非条件Logistic回归分析造成胆管癌患者血清S100A6高水平的危险因素。结果在40例癌旁组织和肝组织中,S100A6蛋白分别阳性8例(20%)和12例(30%),显著低于肝内胆管癌组织的26例(65%,P<0.05);癌旁组织和肝组织S100A6 m RNA相对水平分别为(0.92±0.33)和(1.12±0.46),显著低于肝内胆管癌组织的【(1.93±0.57),P<0.05】;癌旁组织和肝组织S100A6蛋白相对表达量分别为(0.56±0.16)和(0.85±0.46),显著低于肝内胆管癌组织的【(1.63±0.51),P<0.05】;肝内胆管癌患者血清S100A6水平为(2456.85±128.31)pg/ml,显著高于对照组的(2234.26±116.35)pg/ml(P<0.05);S100A6与CEA、CRP、浸润深度、淋巴结转移、TNM分期、肿瘤分化程度呈正相关(P<0.05);多因素Logistic回归分析显示,肿瘤浸润深、淋巴结转移、TNM分期和细胞分化差是造成肿瘤患者血清S100A6高水平的危险因素(P<0.05)。结论血清S100A6高水平的意义还有待于进一步研究。Objective To investigate the relative levels of S100A6 mRNA and its protein expression in tumorous tissues of patients with cholangiocarcinoma.Methods The tumorous tissues in 40 patients with cholangiocarcinoma in our hospital between 2008 and 2014 were collected,and liver tissues in another 40 patients with benign biliary diseases during the same period were also collected as control.S100A6 mRNA and its protein in liver tissues were detected.Serum S100A6 was assayed by ELISA.Univariate and multivariate unconditional Logistic regression analysis was made for the risk factors of high serum S100A6 levels in patients with cholangiocarcinoma.Results The expression of S100A6 protein in tumor adjacent tissues and liver tissues were positive in 8(20%)and in 12(30%),respectively,significantly lower than in 26(65%)in timorous tissues(P<0.05);the relative levels of S100A6 mRNA in adjacent tissues and liver tissues were(0.92±0.33)and(1.12±0.46),significantly lower than(1.93±0.57)in cholangiocarcinoma tissues(P<0.05);the relative expression of S100A6 protein in tumor adjacent tissues and liver tissues was(0.56±0.16)and(0.85±0.46),significantly lower than[(1.63±0.51),P<0.05]in timorous tissues;serum S100A6 level in patients with cholangiocarcinoma was(2456.85±128.31)pg/ml,significantly higher than(2234.26±116.35)pg/ml in control group(P<0.05);serum S100A6 level in patients with cholangiocarcinoma was positively correlated with CEA,CRP,invasion depth,lymph node metastasis,TNM stage and tumor differentiation(P<0.05);multivariate Logistic regression analysis showed that tumor invasion depth,lymph node metastasis,poor TNM stage and differentiation were the risk factors for high serum S100A6 levels in patients with cholangiocarcinoma(P<0.05).Concluson The implication of high serum S100A6 levels in patients with cholangiocarcinoma needs further investigation.

关 键 词：胆管细胞癌 S100A6 危险因素 

分 类 号：R735.8[医药卫生—肿瘤]